Epileptiform curents in rat cortical neurons increase over time of the in vitro culture period
- Авторы: Sibarov D.1, Abushik P.1, Antonov S.1
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Учреждения:
- Sechenov Institute of Evolutionary Physiology and Biochemistry
- Выпуск: Том 10, № 4 (2016)
- Страницы: 338-340
- Раздел: Short Communications
- URL: https://journals.rcsi.science/1990-7478/article/view/213222
- DOI: https://doi.org/10.1134/S1990747816040073
- ID: 213222
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Аннотация
Here we show that in primary culture of rat cortical neurons the number of episodes of epileptiform curents (EC) provoked by extracellular magnesium removal increases over time. We demonstrate that NMDA receptor agonists in low concentrations induce an elevation of frequency of miniature postsynaptic currents followed by their synchronization resulting in EC. Ifenprodil did not block EC but strongly inhibited NMDA-evoked whole-cell currents, which say for a key role of the ifenprodil-resistant synaptic GluN2A-containing NMDA receptors in the generation of EC. We suppose that in cultured neurons the onset of EC and a gradual increase of the EC amplitude over the time of culture period is directed by an increase of synaptic connections density and displacement of the GluN2B subunit by GluN2A in synapses.
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Об авторах
D. Sibarov
Sechenov Institute of Evolutionary Physiology and Biochemistry
Автор, ответственный за переписку.
Email: dsibarov@gmail.com
Россия, pr. Toreza 44, St.-Petersburg, 194223
P. Abushik
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: dsibarov@gmail.com
Россия, pr. Toreza 44, St.-Petersburg, 194223
S. Antonov
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: dsibarov@gmail.com
Россия, pr. Toreza 44, St.-Petersburg, 194223