Epileptiform curents in rat cortical neurons increase over time of the in vitro culture period
- Authors: Sibarov D.A.1, Abushik P.A.1, Antonov S.M.1
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Affiliations:
- Sechenov Institute of Evolutionary Physiology and Biochemistry
- Issue: Vol 10, No 4 (2016)
- Pages: 338-340
- Section: Short Communications
- URL: https://journals.rcsi.science/1990-7478/article/view/213222
- DOI: https://doi.org/10.1134/S1990747816040073
- ID: 213222
Cite item
Abstract
Here we show that in primary culture of rat cortical neurons the number of episodes of epileptiform curents (EC) provoked by extracellular magnesium removal increases over time. We demonstrate that NMDA receptor agonists in low concentrations induce an elevation of frequency of miniature postsynaptic currents followed by their synchronization resulting in EC. Ifenprodil did not block EC but strongly inhibited NMDA-evoked whole-cell currents, which say for a key role of the ifenprodil-resistant synaptic GluN2A-containing NMDA receptors in the generation of EC. We suppose that in cultured neurons the onset of EC and a gradual increase of the EC amplitude over the time of culture period is directed by an increase of synaptic connections density and displacement of the GluN2B subunit by GluN2A in synapses.
Keywords
About the authors
D. A. Sibarov
Sechenov Institute of Evolutionary Physiology and Biochemistry
Author for correspondence.
Email: dsibarov@gmail.com
Russian Federation, pr. Toreza 44, St.-Petersburg, 194223
P. A. Abushik
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: dsibarov@gmail.com
Russian Federation, pr. Toreza 44, St.-Petersburg, 194223
S. M. Antonov
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: dsibarov@gmail.com
Russian Federation, pr. Toreza 44, St.-Petersburg, 194223