Exosomes: Generation, structure, transport, biological activity, and diagnostic application

Resumo

Cells of almost all tissues secrete to the extracellular environment a variety of vesicular structures. The most interesting vesicles are exosomes–microvesicles ranging from 30 to 100 nm in size. These vesicles contain various RNA, including mRNA, microRNA, as well as membrane and cytoplasmic proteins that can be transported in these particles to nearby and distantly located cells of various tissues using physiological fluids (blood, urine, saliva, etc.). Exosomes are necessary for normal functioning of the organism and their repertoire changes during the development of pathologies. This review presents the data on generation, secretion, and transport of exosomes, their structure and roles in normal conditions and in the process of the malignant tumor development. Prospects of the application of exosomal biomarkers for the development of early non-invasive cancer diagnosis are also discussed.