Email this article Applicability of Live Cell Imaging of mRNA Expression in Combination with Calcium Imaging for in vitro Studies of Neural Network Activity
- Authors: Mishchenko T.A.1,2,3, Mitroshina E.V.1,2, Shishkina T.V.1, Astrakhanova T.A.1, Prokhorova M.V.4, Vedunova M.V.1,3
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Affiliations:
- National Research Lobachevsky State University of Nizhni Novgorod
- Nizhny Novgorod State Medical Academy
- Burdenko Research Institute of Neurosurgery
- Merck LLC
- Issue: Vol 12, No 2 (2018)
- Pages: 170-179
- Section: Articles
- URL: https://journals.rcsi.science/1990-7478/article/view/213307
- DOI: https://doi.org/10.1134/S1990747818020095
- ID: 213307
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Abstract
The effectiveness of live cell mRNA detection for neurobiological studies was evaluated. We modified the commercial protocol for the use of RNA detection probes (SmartFlareTM, Merck) for primary hippocampal cultures. It was shown that RNA probes could be used both as an independent evaluation system and in combination with Ca2+ imaging. The complex of these methods provided the unique possibility of performing simultaneous studies of the functional calcium homeostasis of neuronal-glial networks and differential analysis of the plasticity of cells with different levels of mRNA expression.
About the authors
T. A. Mishchenko
National Research Lobachevsky State University of Nizhni Novgorod; Nizhny Novgorod State Medical Academy; Burdenko Research Institute of Neurosurgery
Email: MVedunova@yandex.ru
Russian Federation, Nizhny Novgorod, 603950; Nizhny Novgorod, 603950; Moscow, 125047
E. V. Mitroshina
National Research Lobachevsky State University of Nizhni Novgorod; Nizhny Novgorod State Medical Academy
Email: MVedunova@yandex.ru
Russian Federation, Nizhny Novgorod, 603950; Nizhny Novgorod, 603950
T. V. Shishkina
National Research Lobachevsky State University of Nizhni Novgorod
Email: MVedunova@yandex.ru
Russian Federation, Nizhny Novgorod, 603950
T. A. Astrakhanova
National Research Lobachevsky State University of Nizhni Novgorod
Email: MVedunova@yandex.ru
Russian Federation, Nizhny Novgorod, 603950
M. V. Prokhorova
Merck LLC
Email: MVedunova@yandex.ru
Russian Federation, Moscow, 115054
M. V. Vedunova
National Research Lobachevsky State University of Nizhni Novgorod; Burdenko Research Institute of Neurosurgery
Author for correspondence.
Email: MVedunova@yandex.ru
Russian Federation, Nizhny Novgorod, 603950; Moscow, 125047
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