The mechanism of neuroprotection by positive modulation of Ca2+-activated K+ channels of cerebellar neurons in primary culture
- Authors: Stepanenko Y.D.1, Karelina T.V.1, Sibarov D.A.1, Abushik P.A.1, Antonov S.M.1
-
Affiliations:
- Sechenov Institute of Evolutionary Physiology and Biochemistry
- Issue: Vol 11, No 3 (2017)
- Pages: 248-251
- Section: Short Communication
- URL: https://journals.rcsi.science/1990-7478/article/view/213263
- DOI: https://doi.org/10.1134/S1990747817030084
- ID: 213263
Cite item
Abstract
Here we show that positive modulators (CyPPA and NS309) of Ca2+-activated K+ channels of small (SK) and intermediate (IK) conductances in cerebellar neurons decrease glutamate-evoked Ca2+ entry into neurons independently on the presence of Mg2+ in extracellular media. An analysis of neuronal viability after long-term (240 min) glutamate treatments demonstrated neuroprotective action of CyPPA and NS309. Extracellular Mg2+ did not protect neurons from apoptosis during prolonged treatment with glutamate. Activation of SK and IK channels results in local membrane hyperpolarization, which enhances Mg2+ block of NMDA receptors and reduces activation of voltage-dependent Ca2+ channels, which can explain neuroprotection caused by CyPPA or NS309. The obtained results reveal an important role Ca2+-activated K+ channels of small and intermediate conductance in the regulation of Ca2+ entry into cerebellar neurons via NMDA receptors and voltage-gated Ca2+ channels.
Keywords
About the authors
Yu. D. Stepanenko
Sechenov Institute of Evolutionary Physiology and Biochemistry
Author for correspondence.
Email: juli@unixway.org
Russian Federation, pr. Toreza 44, St. Petersburg, 194223
T. V. Karelina
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: juli@unixway.org
Russian Federation, pr. Toreza 44, St. Petersburg, 194223
D. A. Sibarov
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: juli@unixway.org
Russian Federation, pr. Toreza 44, St. Petersburg, 194223
P. A. Abushik
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: juli@unixway.org
Russian Federation, pr. Toreza 44, St. Petersburg, 194223
S. M. Antonov
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: juli@unixway.org
Russian Federation, pr. Toreza 44, St. Petersburg, 194223