Functional linkage between the transport characteristics of the MDCK1 cell monolayer and their actin cytoskeleton organization

详细

The dynamics of the actin cytoskeleton spatial organization and transepithelial electric resistance (TEER) in the MDCK1 cell monolayer exposed to arginine–vasopressin (AVP) and forskolin, a protein kinase A (PKA) activator, have been studied. These physiologically active substances are shown to depolymerize filamentous actin in MDCK1 cells (in both the apical and basal cytoplasm) and, concurrently, to considerably decrease the TEER of the cell monolayer. A decrease in TEER suggests an increase in the ion current through the cell monolayer. Correspondingly, the created ion gradient stimulates AVP-sensitive water flow. To clarify the routes of ions and water in MDCK monolayer, the localization of claudin-1 and -2 in tight junctions of ATCC (American Type Culture Collection) MDCK (a low TEER) and MDCK1 (a high TEER) cells was studied by immunofluorescence assay. Claudin-1 and -2 are detectable in the tight junctions of ATCC MDCK cells; however, the tight junctions of MDCK1 cells contain only claudin-1, whereas poreforming claudin-2 is absent. The exposure of MDCK1 cells to forskolin fails to change the distribution of the studied claudins, thereby suggesting that a decrease in TEER caused by forskolin is associated with a change in transcellular, rather than paracellular, permeability of the monolayer