The in vitro effect of methylprednisolone on the processes of activation of CD4+CD45RO+ T-cells from healthy subjects and rheumatoid-arthritis patients
- Авторы: Todosenko N.M.1, Khaziakhmatova O.G.1, Yurova K.A.1, Malinina I.P.2, Litvinova L.S.1
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Учреждения:
- Laboratory of Immunology and Cell Biotechnology
- Rheumatology Department
- Выпуск: Том 11, № 6 (2017)
- Страницы: 427-433
- Раздел: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212486
- DOI: https://doi.org/10.1134/S1990519X17060104
- ID: 212486
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Аннотация
Flow cytometry has been used to analyze the changes in the number of CD4+ cells that expressed surface markers of activation (CD25, CD71, HLA-DR, and CD95) in cultures of TCR-stimulated CD3+CD45RO+ Т-lymphocytes after in vivo exposure to different concentrations of methylprednisolone (MP). T-cells were obtained from healthy donors and rheumatoid arthritis (RA) patients. Suppressive action of МР on the expression of activation and proliferation markers (CD25 and CD71, respectively) by CD4+ T-cells was observed in all study subjects. МР increased the number of CD4+ HLA-DR+/CD95+ cells among the СD3+CD45RO+ cells obtained from RA patients and subjected to TCR activation, whereas the number of such cells in the control group decreased after MP treatment. The MP-induced changes in the cells subjected to TCR activation can be indicative of relative resistance of the CD4+CD45RO+HLA-DR+/CD95+ cell population in RA patients to the action of glucocorticoids and the possible role of this subpopulation in RA pathogenesis.
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Об авторах
N. Todosenko
Laboratory of Immunology and Cell Biotechnology
Email: larisalitvinova@yandex.ru
Россия, Kaliningrad, 236016
O. Khaziakhmatova
Laboratory of Immunology and Cell Biotechnology
Email: larisalitvinova@yandex.ru
Россия, Kaliningrad, 236016
K. Yurova
Laboratory of Immunology and Cell Biotechnology
Email: larisalitvinova@yandex.ru
Россия, Kaliningrad, 236016
I. Malinina
Rheumatology Department
Email: larisalitvinova@yandex.ru
Россия, Kaliningrad, 236019
L. Litvinova
Laboratory of Immunology and Cell Biotechnology
Автор, ответственный за переписку.
Email: larisalitvinova@yandex.ru
Россия, Kaliningrad, 236016
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