Intermolecular interactions in solutions of serum albumin
- Авторлар: Polyanichko A.1,2, Mikhailov N.1, Romanov N.1, Baranova Y.1, Chikhirzhina E.2
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Мекемелер:
- St. Petersburg State University
- Institute of Cytology
- Шығарылым: Том 11, № 1 (2017)
- Беттер: 9-15
- Бөлім: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212235
- DOI: https://doi.org/10.1134/S1990519X17010084
- ID: 212235
Дәйексөз келтіру
Аннотация
The mechanisms of intermolecular protein complex formation were studied by the example of monomers, oligomers and aggregates of bovine serum albumin (BSA) depending on the protein concentration, pH and urea concentration. Using dynamic light scattering (DLS), analytical ultracentrifugation (AUC) and PAG electrophoresis we have shown the existence of dynamic equilibrium between monomers and aggregates in BSA solution. Decreasing pH of the solution (4.0–1.0) resulted in increasing sizes of the aggregates. In the solutions with low urea concentrations (below 2 M) the sizes of aggregates decreased, while higher urea concentrations (2–8 M) induced formation of larger aggregates due to the unfolding of the protein.
Негізгі сөздер
Авторлар туралы
A. Polyanichko
St. Petersburg State University; Institute of Cytology
Хат алмасуға жауапты Автор.
Email: a.polyanichko@spbu.ru
Ресей, St. Petersburg, 199034; St. Petersburg, 194064
N. Mikhailov
St. Petersburg State University
Email: a.polyanichko@spbu.ru
Ресей, St. Petersburg, 199034
N. Romanov
St. Petersburg State University
Email: a.polyanichko@spbu.ru
Ресей, St. Petersburg, 199034
Yu. Baranova
St. Petersburg State University
Email: a.polyanichko@spbu.ru
Ресей, St. Petersburg, 199034
E. Chikhirzhina
Institute of Cytology
Email: a.polyanichko@spbu.ru
Ресей, St. Petersburg, 194064