Heterogeneity of coilin-containing nuclear domains in early mouse embryos
- Authors: Bogolyubova I.O.1
-
Affiliations:
- Institute of Cytology
- Issue: Vol 11, No 4 (2017)
- Pages: 293-299
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212411
- DOI: https://doi.org/10.1134/S1990519X17040034
- ID: 212411
Cite item
Abstract
The nucleus of two-cell mouse embryos contains coilin-containing bodies of two types: (i) one to three large spherical structures 1 μm in size and (ii) small foci, which vary in number in different blastomeres. The largest coilin-containing structures, unlike the smallest ones, contain RNA polymerase I, nucleic acid chaperon YB-1, and actin. Neither large nor small coilin-positive domains contain symplekin, one of the signature components of histone locus bodies. In nuclei of late two-cell embryos, symplekin localizes to one to two well-formed spherical bodies that are observed both in close proximity to the coilin-positive structures and apart from them. Large coilin-containing bodies were not observed in embryos at the morula stage, as well as in the nuclei of late two-cell embryos after artificial suppression of transcription activity. Thus, the population of coilin-containing bodies in the nuclei of late two-cell embryos of mice is heterogeneous in morphology and molecular composition. It can be suggested that the largest coilin-containing bodies are provisional nuclear domains that are formed against a background of significant changes in the nuclear metabolism at the final stages of embryonic genome activation and the initial stages of reactivation of nucleolar transcription.
About the authors
I. O. Bogolyubova
Institute of Cytology
Author for correspondence.
Email: ibogol@mail.ru
Russian Federation, St. Petersburg, 194064