Additional Prognostic Markers of Human Colon Adenocarcinoma
- Authors: Bystriakova M.A.1, Koshkin S.A.1, Gavrilyukov A.V.2, Tolkunova E.N.1
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Affiliations:
- Institute of Cytology, Russian Academy of Sciences
- St. Petersburg Clinical Scientific and Practical Center of Specialized Medical Care (Oncology-Oriented)
- Issue: Vol 13, No 1 (2019)
- Pages: 31-41
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212842
- DOI: https://doi.org/10.1134/S1990519X19010048
- ID: 212842
Cite item
Abstract
The expression of potential prognostic markers of human colon adenocarcinoma in tumor and adjacent normal tissue, as well as the relationship between their expression levels was studied. The expression of potential prognostic markers (IGFBP3, AhR, CYP1A1, CYP1B1, HIF-1α, OCT4A, OCT4B, OCT4B1) was assayed with quantitative RT-PCR (qPCR). A correlation between IGFBP3 and AhR expression was found. AhR signaling was analyzed in the primary culture of human colon adenocarcinoma BSK 8 with IGFBP3 gene knockout. It was found that the enhanced expression of AhR can be an additional diagnostic criterion for colon cancer. The correlation between the expression level of HIF-1α, IGFPB3, and OCT4 isoforms and clinic stage (TNM) was not estimated. Clones with IGFBP3 gene knockout had augmented doubling time and increased resistance to the cytostatic drug 5-ftoruracile after modulated activity of dioxin receptor with its agonist—indol-3-carbinol.
About the authors
M. A. Bystriakova
Institute of Cytology, Russian Academy of Sciences
Email: entolk62@mail.ru
Russian Federation, St. Petersburg, 194064
S. A. Koshkin
Institute of Cytology, Russian Academy of Sciences
Email: entolk62@mail.ru
Russian Federation, St. Petersburg, 194064
A. V. Gavrilyukov
St. Petersburg Clinical Scientific and Practical Center of Specialized Medical Care (Oncology-Oriented)
Email: entolk62@mail.ru
Russian Federation, St. Petersburg, 197758
E. N. Tolkunova
Institute of Cytology, Russian Academy of Sciences
Author for correspondence.
Email: entolk62@mail.ru
Russian Federation, St. Petersburg, 194064