Peculiarities of Phagocytosis of Opsonized and Nonopsonized Bacteria S. Aureus and E. Coli by Human Neutrophil Granulocytes Studied by Atomic Force Microscopy
- Authors: Pleskova S.N.1,2, Kriukov R.N.1, Razumkova E.V.1,2, Zubkov S.Y.1, Abarbanel N.V.1,2
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Affiliations:
- Scientific and Educational Center for Physics of Solid-State Nanostructures, Lobachevsky State University of Nizhny Novgorod
- Nanotechnology and Biotechnology Department, Alekseev Nizhny Novgorod State Technical University
- Issue: Vol 12, No 6 (2018)
- Pages: 496-505
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212813
- DOI: https://doi.org/10.1134/S1990519X18060081
- ID: 212813
Cite item
Abstract
Differences in the phagocytosis process of opsonized and nonopsonized strains of Staphylococcus aureus 2879 M and Escherichia coli 321 were studied. Differences in the character of pseudopodia during phagocytosis by neutrophil granulocytes (NGs) of opsonized and nonopsonized bacteria were detected, and differences in the nature of pseudopodia in reactions to gram-positive and gram-negative microorganisms were not detected. For the first time in dynamic observations at the late stages of phagocytosis, changes in the volume of nuclei and their movement, variations in the intersegment distance of the nuclei, and a slight increase in the volume of NGs were shown. A decrease in the rigidity of the membrane–cytoskeleton NG complex correlating with the intensity of phagocytosis and opsonization of bacteria was shown for the first time. It was established that opsonization does not affect the oxygen-dependent metabolism of NGs and, at the same time, introduces significant adjustments in the implementation of oxygen-independent bactericidal mechanisms of cells.
About the authors
S. N. Pleskova
Scientific and Educational Center for Physics of Solid-State Nanostructures,Lobachevsky State University of Nizhny Novgorod; Nanotechnology and Biotechnology Department, Alekseev Nizhny Novgorod State Technical University
Author for correspondence.
Email: pleskova@mail.ru
Russian Federation, Nizhny Novgorod, 603950;
Nizhny Novgorod, 603950
R. N. Kriukov
Scientific and Educational Center for Physics of Solid-State Nanostructures,Lobachevsky State University of Nizhny Novgorod
Email: pleskova@mail.ru
Russian Federation, Nizhny Novgorod, 603950
E. V. Razumkova
Scientific and Educational Center for Physics of Solid-State Nanostructures,Lobachevsky State University of Nizhny Novgorod; Nanotechnology and Biotechnology Department, Alekseev Nizhny Novgorod State Technical University
Email: pleskova@mail.ru
Russian Federation, Nizhny Novgorod, 603950;
Nizhny Novgorod, 603950
S. Yu. Zubkov
Scientific and Educational Center for Physics of Solid-State Nanostructures,Lobachevsky State University of Nizhny Novgorod
Email: pleskova@mail.ru
Russian Federation, Nizhny Novgorod, 603950
N. V. Abarbanel
Scientific and Educational Center for Physics of Solid-State Nanostructures,Lobachevsky State University of Nizhny Novgorod; Nanotechnology and Biotechnology Department, Alekseev Nizhny Novgorod State Technical University
Email: pleskova@mail.ru
Russian Federation, Nizhny Novgorod, 603950;
Nizhny Novgorod, 603950