Inhibitors of the Metabolism of Arachidonic Acid Suppress Ca2+ Responses Induced by Trifluoperazine in Macrophages
- Authors: Milenina L.S.1, Krutetskaya Z.I.1, Naumova A.A.1, Butov S.N.1, Krutetskaya N.I.1, Antonov V.G.1
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Affiliations:
- Department of Biophysics
- Issue: Vol 12, No 4 (2018)
- Pages: 315-322
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212716
- DOI: https://doi.org/10.1134/S1990519X18040065
- ID: 212716
Cite item
Abstract
The influence of the neuroleptic trifluoperazine on the intracellular concentration of Ca2+ in macrophages of rats was studied using a Fura-2AM fluorescent Ca2+ probe. It was found that trifluoperazine causes a dose-dependent increase in the intracellular Ca2+ concentration associated with Ca2+ mobilization from intracellular Ca2+ stores and subsequent entry of Ca2+ into peritoneal macrophages of rats. It was also shown that inhibitors of phospholipase A2 (4-bromophenacyl bromide, prednisolone, and dexamethasone), cyclooxygenases (aspirin and indomethacin), and lipoxygenases (caffeic acid, zileuton, and baicalein) suppress Ca2+ responses induced by trifluoperazine in macrophages. The data obtained indicate the participation of enzymes and/or products of the cascade of arachidonic acid metabolism in the influence of trifluoperazine on the intracellular concentration of Ca2+ in peritoneal macrophages.
About the authors
L. S. Milenina
Department of Biophysics
Author for correspondence.
Email: cozzy@mail.ru
Russian Federation, St. Petersburg, 199034
Z. I. Krutetskaya
Department of Biophysics
Email: cozzy@mail.ru
Russian Federation, St. Petersburg, 199034
A. A. Naumova
Department of Biophysics
Email: cozzy@mail.ru
Russian Federation, St. Petersburg, 199034
S. N. Butov
Department of Biophysics
Email: cozzy@mail.ru
Russian Federation, St. Petersburg, 199034
N. I. Krutetskaya
Department of Biophysics
Email: cozzy@mail.ru
Russian Federation, St. Petersburg, 199034
V. G. Antonov
Department of Biophysics
Email: cozzy@mail.ru
Russian Federation, St. Petersburg, 199034