Fluorene Derivative Disodium Salt as a New Fluorescent Dye for Identification of Amyloid Deposits in Myocardium of mdx Mice
- Authors: Gusel’nikova V.V.1, Antimonova O.I.1,2, Fedorova E.A.1, Shavlovskii M.M.1,3,4,5, Krutikov A.N.2, Mikhailova E.V.4, Kaminskaya E.V.4, Gudkova A.Y.2,3, Korzhevskii D.E.1,3, Mikhailov V.M.4
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Affiliations:
- Institute of Experimental Medicine
- Almazov Northwestern Federal Medical Research Center
- St. Petersburg Pavlov State Medical University
- Institute of Cytology
- Northwestern State Medical University Named after I.I. Mechnikov
- Issue: Vol 12, No 3 (2018)
- Pages: 213-216
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212656
- DOI: https://doi.org/10.1134/S1990519X18030057
- ID: 212656
Cite item
Abstract
The aim of this study was to develop a new method for the detection of amyloid deposits in laboratory animals using an analogue of Congo red synthesized on the basis of diaminofluorene. The analogue is disodium salt of 2,7-(1-amino-4-sulfo-2-naphthylazo)fluorene (DSNAF). Myocardial samples from mdx mice of both sexes aged 1–1.5 years (n = 8) were used as the material for this study. The main result of this study was the development of an optimal protocol for amyloid staining with DSNAF. It has been shown that the sensitivity and specificity of amyloid detection by this method is comparable with Congo-red staining. The clear advantages of using DSNAF are stability of staining, high fluorescence intensity of amyloid deposits, and total lack of background fluorescence, which greatly simplifies the procedure of quantitative evaluation of obtained results. The method of amyloid staining with DSNAF is characterized by simplicity and good reproducibility. Further research will show the possibility to apply this method for diagnosis of amyloidosis in the practice of clinical research.
About the authors
V. V. Gusel’nikova
Institute of Experimental Medicine
Author for correspondence.
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197376
O. I. Antimonova
Institute of Experimental Medicine; Almazov Northwestern Federal Medical Research Center
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197376; St. Petersburg, 197341
E. A. Fedorova
Institute of Experimental Medicine
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197376
M. M. Shavlovskii
Institute of Experimental Medicine; St. Petersburg Pavlov State Medical University; Institute of Cytology; Northwestern State Medical University Named after I.I. Mechnikov
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197376; St. Petersburg, 197022; St. Petersburg, 194064; St. Petersburg, 195067
A. N. Krutikov
Almazov Northwestern Federal Medical Research Center
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197341
E. V. Mikhailova
Institute of Cytology
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 194064
E. V. Kaminskaya
Institute of Cytology
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 194064
A. Ya. Gudkova
Almazov Northwestern Federal Medical Research Center; St. Petersburg Pavlov State Medical University
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197341; St. Petersburg, 197022
D. E. Korzhevskii
Institute of Experimental Medicine; St. Petersburg Pavlov State Medical University
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197376; St. Petersburg, 197022
V. M. Mikhailov
Institute of Cytology
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 194064