Behavioral Changes of Multipotent Mesenchymal Stromal Cells in Contact with Synthetic Calcium Phosphates in vitro
- Authors: Litvinova L.S.1, Shupletsova V.V.1, Khaziakhmatova O.G.1, Yurova K.A.1, Malashchenko V.V.1, Melashchenko E.S.1, Todosenko N.M.1, Khlusova M.Y.2, Sharkeev Y.P.3,4, Komarova E.G.4, Sedelnikova M.B.4, Shunkin E.O.1, Khlusov I.A.1,5
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Affiliations:
- Laboratory of Immunology and Cell Biotechnology
- Pathophysiology Department
- Experimental Physics Department
- Institute of Strength Physics and Materials Science of the Siberian Branch of the Russian Academy of Sciences
- Morphology and General Pathology Department
- Issue: Vol 12, No 2 (2018)
- Pages: 112-119
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212603
- DOI: https://doi.org/10.1134/S1990519X18020062
- ID: 212603
Cite item
Abstract
Migration, proliferation, and osteogenic differentiation of human adipose-derived (AD) multipotent mesenchymal stromal cells (MMSCs) during in vitro modeling of indirect contact with calcium phosphate (CP) or nanoparticles of synthetic hydroxyapatite (HA) have been studied. The results were registered with electrode (real-time cell analysis, RTCA) or visual (Cell-IQ) systems of long-term observation of cell cultures. Bulk specimens were use in a Cell-IQ® v2 MLF device as pure titanium substrates (10 × 10 × 1 mm3) covered by a CP relief (roughness index Ra = 2.4–4.4 μm) bilateral coating that was prepared by the micr-arc method from an aqueous solution of orthophosphoric acid (20 wt %), calcium carbonate (9 wt %), and synthetic HA (6 wt %). HA crystallites (1 mg/mL) were fabricated by mechanochemical synthesis and served as an irritant in RTCA investigation. The Cell-IQ system identified a 3.5- to 10-fold decrease in cell number at the interface with CP coatings with differing roughness during 14-day cell culturing. After 21 days, it was accompanied by a weak reduction of MMSC antigen expression (CD73, CD90, and CD105) as opposed to an increase in MMSC osteogenic differentiation and intercellular-matrix mineralization. In turn, HA nanodispersion reduced the speed of MMSC migration by 1.5 times (P < 0.001) during 25-h RTCA recording, which simulated cell invasion through the microporous membrane (8-μm diameter). Inhibition of migration and cell division with increased osteogenic differentiation of MMSCs has been suggested to be a possible effect of biodegradation products of synthetic CP materials.
About the authors
L. S. Litvinova
Laboratory of Immunology and Cell Biotechnology
Author for correspondence.
Email: larisalitvinova@yandex.ru
Russian Federation, Kaliningrad, 236041
V. V. Shupletsova
Laboratory of Immunology and Cell Biotechnology
Email: larisalitvinova@yandex.ru
Russian Federation, Kaliningrad, 236041
O. G. Khaziakhmatova
Laboratory of Immunology and Cell Biotechnology
Email: larisalitvinova@yandex.ru
Russian Federation, Kaliningrad, 236041
K. A. Yurova
Laboratory of Immunology and Cell Biotechnology
Email: larisalitvinova@yandex.ru
Russian Federation, Kaliningrad, 236041
V. V. Malashchenko
Laboratory of Immunology and Cell Biotechnology
Email: larisalitvinova@yandex.ru
Russian Federation, Kaliningrad, 236041
E. S. Melashchenko
Laboratory of Immunology and Cell Biotechnology
Email: larisalitvinova@yandex.ru
Russian Federation, Kaliningrad, 236041
N. M. Todosenko
Laboratory of Immunology and Cell Biotechnology
Email: larisalitvinova@yandex.ru
Russian Federation, Kaliningrad, 236041
M. Yu. Khlusova
Pathophysiology Department
Email: larisalitvinova@yandex.ru
Russian Federation, Tomsk, 634050
Yu. P. Sharkeev
Experimental Physics Department; Institute of Strength Physics and Materials Science of the Siberian Branch of the Russian Academy of Sciences
Email: larisalitvinova@yandex.ru
Russian Federation, Tomsk, 634050; Tomsk, 634055
E. G. Komarova
Institute of Strength Physics and Materials Science of the Siberian Branch of the Russian Academy of Sciences
Email: larisalitvinova@yandex.ru
Russian Federation, Tomsk, 634055
M. B. Sedelnikova
Institute of Strength Physics and Materials Science of the Siberian Branch of the Russian Academy of Sciences
Email: larisalitvinova@yandex.ru
Russian Federation, Tomsk, 634055
E. O. Shunkin
Laboratory of Immunology and Cell Biotechnology
Email: larisalitvinova@yandex.ru
Russian Federation, Kaliningrad, 236041
I. A. Khlusov
Laboratory of Immunology and Cell Biotechnology; Morphology and General Pathology Department
Email: larisalitvinova@yandex.ru
Russian Federation, Kaliningrad, 236041; Tomsk, 634050