Human IL-36RA production in Escherichia coli with coexpression of E. coli methionine aminopeptidase. I. Comparison of IL-36RA production in different strains
- Authors: Kolobov A.A.1,2, Kondratyeva E.V.2, Kudling T.V.2, Karasev M.M.2, Kalinin R.S.1,2, Khizhina A.A.1,2, Nimiritsky P.P.1, Stefanov V.E.1, Petrov A.V.2
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Affiliations:
- Department of Biochemistry
- Institute of Highly Pure Biopreparations
- Issue: Vol 11, No 6 (2017)
- Pages: 447-452
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212498
- DOI: https://doi.org/10.1134/S1990519X17060062
- ID: 212498
Cite item
Abstract
Generalized pustular psoriasis (GPP) is a rare, sometimes lethal, form of psoriasis caused by series of mutations in the interleukin-36 receptor antagonist (IL-36RA) gene associated with its reduced expression or activity. Administration of exogenous IL-36RA can be a potent therapeutic approach to treating GPP and other forms of psoriasis. Since cleavage of the starting N-formylmethionine residue from the N-terminal end is needed for full biological activity of IL-36RA, we have developed a technique for producing IL-36RA lacking N-formylmethionine residue in E. coli. We have created a series of plasmids carrying the E. coli methionine aminopeptidase (MAP) gene under the control of different promoters for coexpression of IL-36RA and MAP and tested their effect on IL-36RA production. The highest production of IL-36RA with <3% of unprocessed molecules with uncleaved N-terminal formylmethionine residue has been shown for E. coli strain carrying the MAP gene under the control of arabinose-inducible promoter.
About the authors
A. A. Kolobov
Department of Biochemistry; Institute of Highly Pure Biopreparations
Author for correspondence.
Email: alexey.kolobov.spb@gmail.com
Russian Federation, St. Petersburg, 199004; St. Petersburg, 197110
E. V. Kondratyeva
Institute of Highly Pure Biopreparations
Email: alexey.kolobov.spb@gmail.com
Russian Federation, St. Petersburg, 197110
T. V. Kudling
Institute of Highly Pure Biopreparations
Email: alexey.kolobov.spb@gmail.com
Russian Federation, St. Petersburg, 197110
M. M. Karasev
Institute of Highly Pure Biopreparations
Email: alexey.kolobov.spb@gmail.com
Russian Federation, St. Petersburg, 197110
R. S. Kalinin
Department of Biochemistry; Institute of Highly Pure Biopreparations
Email: alexey.kolobov.spb@gmail.com
Russian Federation, St. Petersburg, 199004; St. Petersburg, 197110
A. A. Khizhina
Department of Biochemistry; Institute of Highly Pure Biopreparations
Email: alexey.kolobov.spb@gmail.com
Russian Federation, St. Petersburg, 199004; St. Petersburg, 197110
P. P. Nimiritsky
Department of Biochemistry
Email: alexey.kolobov.spb@gmail.com
Russian Federation, Moscow, 119192
V. E. Stefanov
Department of Biochemistry
Email: alexey.kolobov.spb@gmail.com
Russian Federation, St. Petersburg, 199004
A. V. Petrov
Institute of Highly Pure Biopreparations
Email: alexey.kolobov.spb@gmail.com
Russian Federation, St. Petersburg, 197110