A truncated form of α-tubulin detected in purified proteasome complexes
- Authors: Ivanova E.Y.1,2, Artamonova T.O.3, Zaikova Y.Y.2, Khodorkovskii M.A.3, Tsimokha A.S.2
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Affiliations:
- St. Petersburg State University
- Institute of Cytology
- Nanobiotechnogy Center
- Issue: Vol 11, No 3 (2017)
- Pages: 191-196
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212347
- DOI: https://doi.org/10.1134/S1990519X1703004X
- ID: 212347
Cite item
Abstract
The 26S proteasome is a multisubunit protein complex responsible for selective protein degradation in the cell. A number of proteins with known and unknown functions were shown to be permanently or temporarily associated with 26S proteasomes. Identification of proteins that interact with proteasomes is an important step in the understanding of the proteasome functions in the cell and the mechanisms of their regulation. Using MALDI–ICR mass spectrometry, we have shown that some proteins of the cytoskeleton, such as actin, α-actinin 4, and α- and β-tubulins are associated with proteasomes obtained by affinity purification from the human myelogenous leukemia cell line K562. Western blot analysis showed that a truncated form of α-tubulin was associated with the purified proteasomes. The presence of the α-tubulin isoform in complex with affinity purified proteasomes was also observed in the human embryonic kidney cell line 293.
About the authors
E. Yu. Ivanova
St. Petersburg State University; Institute of Cytology
Author for correspondence.
Email: ivanoval.027@gmail.com
Russian Federation, St. Petersburg, 199034; St. Petersburg, 194064
T. O. Artamonova
Nanobiotechnogy Center
Email: atsimokha@incras.ru
Russian Federation, St. Petersburg, 195251
Yu. Ya. Zaikova
Institute of Cytology
Email: atsimokha@incras.ru
Russian Federation, St. Petersburg, 194064
M. A. Khodorkovskii
Nanobiotechnogy Center
Email: atsimokha@incras.ru
Russian Federation, St. Petersburg, 195251
A. S. Tsimokha
Institute of Cytology
Author for correspondence.
Email: atsimokha@incras.ru
Russian Federation, St. Petersburg, 194064