Establishment and characterization of clonal lines with cancer stem- and progenitor-cell properties from monolayer Zajdela hepatoma
- Authors: Teryukova N.P.1, Sakhenberg E.I.1, Ivanov V.A.1, Snopov S.A.1
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Affiliations:
- Institute of Cytology
- Issue: Vol 11, No 2 (2017)
- Pages: 161-171
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212333
- DOI: https://doi.org/10.1134/S1990519X17020079
- ID: 212333
Cite item
Abstract
The aim of this study was to investigate the biology of cancer stem cells (CSC) from a metastatic tumor. Previously, we explanted the cells of rat ascites Zajdela hepatoma in vitro. We established a permanent monolayer cell line via selection of adhesive cells from multicellular floating islets. In the present work, we cloned these cells by a limiting-dilution method and established five novel clonal sublines of the hepatoma: three holoclonal sublines containing CSC and two meroclonal sublines. After a long-term cultivation (approximately 30 passages, freezing, and thawing), the cell of clonal sublines retained the features of CSC. They have a tumor-initiating potential and produce mainly holoclones upon recloning in the complete growth medium and large nonadhesive hepatospheres in the serum-free medium. Morphometric analysis showed that the cells of holoclonal and meroclonal sublines differed in the cell shape, area, nucleus size, and nuclear-cytoplasmic ratio. We have found for the first time that holoclonal cells of Zajdela hepatoma have a fibroblast-like morphology and form contacts with each other due to membrane protrusions. We suggest that the fibroblast-like morphology of CSC is an attribute of a metastatic tumor and demonstrates the capability of these cells for individual migration.
Keywords
About the authors
N. P. Teryukova
Institute of Cytology
Author for correspondence.
Email: npter@yandex.ru
Russian Federation, St. Petersburg, 194064
E. I. Sakhenberg
Institute of Cytology
Email: snopov@incras.ru
Russian Federation, St. Petersburg, 194064
V. A. Ivanov
Institute of Cytology
Email: snopov@incras.ru
Russian Federation, St. Petersburg, 194064
S. A. Snopov
Institute of Cytology
Author for correspondence.
Email: snopov@incras.ru
Russian Federation, St. Petersburg, 194064