Characterization of amyloid deposits found in internal organs of mdx mice
- Authors: Gusel’nikova V.V.1, Gudkova A.Y.2,3, Semernin E.N.2,3, Grudinin N.A.1,2, Krutikov A.N.2, Shavloskii M.M.1, Mil’man B.L.3, Korzhevskii D.E.1,3, Mikhailova E.V.4, Kaminskaya E.V.4, Mikhailov V.M.4
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Affiliations:
- Institute of Experimental Medicine
- Almazov Northwestern Federal Medical Research Center
- St. Petersburg Pavlov State Medical University
- Institute of Cytology
- Issue: Vol 11, No 1 (2017)
- Pages: 27-34
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212242
- DOI: https://doi.org/10.1134/S1990519X17010047
- ID: 212242
Cite item
Abstract
The mdx mouse strain is the most widely used experimental model of Duchenne muscular dystrophy (DMD). Although it was previously shown that muscle biopsy specimens obtained from patients with different types of muscular dystrophy contain amyloid, no available publications have analyzed the presence of amyloid aggregates in tissues of DMD patients or mdx mice. The objective of the present work was to verify whether the internal organs of mdx mice might accumulate amyloid. The study was performed in the myocardium, kidney, and liver specimens obtained from male and female mdx mice aged from 2 to 18 months. Using histochemical staining with Congo red, amyloid aggregates were detected in mouse organs studied, and their morphology and location were analyzed. Mass spectrometry data suggest that the most probable components of amyloid aggregates found in mdx mice are vitronectin and apolipoprotein A-II.
Keywords
About the authors
V. V. Gusel’nikova
Institute of Experimental Medicine
Author for correspondence.
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197376
A. Ya. Gudkova
Almazov Northwestern Federal Medical Research Center; St. Petersburg Pavlov State Medical University
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197341; St. Petersburg, 197022
E. N. Semernin
Almazov Northwestern Federal Medical Research Center; St. Petersburg Pavlov State Medical University
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197341; St. Petersburg, 197022
N. A. Grudinin
Institute of Experimental Medicine; Almazov Northwestern Federal Medical Research Center
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197376; St. Petersburg, 197341
A. N. Krutikov
Almazov Northwestern Federal Medical Research Center
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197341
M. M. Shavloskii
Institute of Experimental Medicine
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197376
B. L. Mil’man
St. Petersburg Pavlov State Medical University
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197022
D. E. Korzhevskii
Institute of Experimental Medicine; St. Petersburg Pavlov State Medical University
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 197376; St. Petersburg, 197022
E. V. Mikhailova
Institute of Cytology
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 194064
E. V. Kaminskaya
Institute of Cytology
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 194064
V. M. Mikhailov
Institute of Cytology
Email: Guselnicova.Valeriia@yandex.ru
Russian Federation, St. Petersburg, 194064