An increase in model lipid membrane fluidity as a result of local anesthetic action
- Authors: Efimova S.S.1, Medvedev R.Y.1, Schagina L.V.1, Ostroumova O.S.1
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Affiliations:
- Institute of Cytology
- Issue: Vol 10, No 4 (2016)
- Pages: 324-331
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212143
- DOI: https://doi.org/10.1134/S1990519X16040052
- ID: 212143
Cite item
Abstract
The effect of local anesthetics on the permeability of phospholipid liposomes of different composition for calcein has been investigated. The local anesthetics tested included amides (lidocaine, prilocaine, mepivacaine, and bupivacaine) and esters (benzocaine, procaine, and tetracaine). The permeability of large monolamellar liposomes was assessed by monitoring the fluorescence of calcein leaking from the phospholipid vesicles. All tested amide anesthetics exerted negligible effects on the permeability of dioleylphosphocholine (DOPC) liposomes for the fluorescent marker. The most efficient in this group was did bupivacaine. Amides had a more pronounced effect on membranes in which 20 mol % of DOPC was replaced by tetraoleoylcardiolipin (TOCL). Benzocaine and procaine at concentration up to 100 mM did not affect the permeability of DOPC liposomes. Membrane permeability of DOPC liposomes was not affected by the addition of tetracaine to the final concentration of 2 mM, while the increase of anesthetic concentration up to 50 mM was accompanied by an increase in the intensity of fluorescence of calcein released from the vesicles, and addition of the anesthetic to the concentration of 100 mM caused by complete release of the marker incorporated by the liposomes. The threshold concentration of tetracaine initiating calcein leakage from vesicles that contained 20 mol % TOCL was 7 mM, and the concentration corresponding to 100% calcein leakage was 20 mM. Confocal fluorescence microscopy of giant monolamellar liposomes formed from an equimolar mixture of DOPC and tetramiristoylcardiolipin demonstrated the destruction of solid ordered domains at the presence of anesthetics, and its destructive capacity increasing in the following order: procaine ≈ mepivacaine < bupivacaine ≪ tetracaine. Variability of the depth of anesthetic incorporation into the membrane may account for the dissimilar effects of local anesthetics on liposomes.
About the authors
S. S. Efimova
Institute of Cytology
Author for correspondence.
Email: ssefimova@mail.ru
Russian Federation, St. Petersburg
R. Ya. Medvedev
Institute of Cytology
Email: ssefimova@mail.ru
Russian Federation, St. Petersburg
L. V. Schagina
Institute of Cytology
Email: ssefimova@mail.ru
Russian Federation, St. Petersburg
O. S. Ostroumova
Institute of Cytology
Email: ssefimova@mail.ru
Russian Federation, St. Petersburg