Oxidative stress response of human fibroblasts and endometrial mesenchymal stem cells
- Authors: Shatrova A.N.1, Lyublinskaya O.G.1, Borodkina A.V.1, Burova E.B.1
-
Affiliations:
- Institute of Cytology
- Issue: Vol 10, No 1 (2016)
- Pages: 18-28
- Section: Article
- URL: https://journals.rcsi.science/1990-519X/article/view/212015
- DOI: https://doi.org/10.1134/S1990519X16010090
- ID: 212015
Cite item
Abstract
Human mesenchymal stem cells are a promising cell source for tissue engineering. During transplantation, they may be subjected to oxidative stress due to unfavorable cellular microenvironment characterized by an increased level of reactive oxygen species. Recently, we have demonstrated that oxidative stress response of human mesenchymal stem cells derived from endometrium (hMESCs) depends on the oxidizer concentration. The duration of cell treatment with an oxidizer also may play an important role. In this study, we investigated the dependence of the cell response on H2O2 treatment duration. The effects of high H2O2 doses on hMESCs and human lung embryonic fibroblasts were compared. In both cell types, H2O2 treatment for 60 min caused multiphase cell cycle arrest, with dose-dependent cell death occurring equally in all phases of the cell cycle. However, the cell death dynamics in hMESCs and fibroblasts were different. Interestingly, in both cell types, shortening of H2O2 treatment from 60 to 10 min induced growth retardation, G1-phase cell accumulation, and cell size increase. Collectively, these findings suggest that there is induction of premature senescence. Thus, shortening of oxidative stress induced in human endometrial stem cells and embryonic fibroblasts by high H2O2 doses enables one to modulate cellular response as both cell death and premature senescence.
About the authors
A. N. Shatrova
Institute of Cytology
Author for correspondence.
Email: Shatrova@mail.ru
Russian Federation, St. Petersburg, 194064
O. G. Lyublinskaya
Institute of Cytology
Email: Shatrova@mail.ru
Russian Federation, St. Petersburg, 194064
A. V. Borodkina
Institute of Cytology
Email: Shatrova@mail.ru
Russian Federation, St. Petersburg, 194064
E. B. Burova
Institute of Cytology
Email: Shatrova@mail.ru
Russian Federation, St. Petersburg, 194064