The effects of isoquinoline carboxamide and melatonin on the differentiation of N1Е-115 mouse neuroblastoma cells (clone C-1300) and on the expression of the TSPO translocation protein and 2’,3’-cyclonucleotide-3’-phosphodiesterase in these cells

Abstract

We studied the effect of isoquinoline carboxamide (PK11195) applied alone or in combination with melatonin on the differentiation of N1E-115 mouse neuroblastoma cells (clone C-1300). PK11195 is a synthetic ligand of the mitochondrial translocator protein (TSPO), which is one of the mitochondrial proteins that are responsible for the opening of the mitochondrial pore (mPTP); expression of this protein is enhanced in different types of cancer cells. PK11195 is considered as a potential anticancer drug. It has been shown that PK11195 at a nontoxic/subtoxic concentration induces the differentiation of the N1Е-115 mouse neuroblastoma cells and suppresses cell proliferation; the magnitude of this effect coincides with the effect induced by melatonin at a nontoxic concentration. The combination of PK11195 with melatonin did not intensify its effect as a differentiation inducer. Based on the results of Western blot analysis, it has been hypothesized that the differentiation of N1Е-115 neuroblastoma cells is associated with the expression of mitochondrial 2’,3’-cyclonucleotide-3’-phosphodiesterase but not with the expression of the mitochondrial translocator protein TSPO.