Dynamics of circulating tumor cells during neoadjuvant chemotherapy in patients with locally-advanced breast cancer


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Abstract Relevance. Circulating tumor cells (CTC) are a biological marker of new generation, which provides additional information on the biological features and prognosis of the disease. The possibility of using CTC for early evaluation of the effectiveness of drug treatment of breast cancer (BC), including neoadjuvant chemotherapy, is an important but insufficiently studied issue. Goal - to study the dynamics of CTCs in the process of preoperative chemotherapy in patients with locally-advanced BC. Materials and methods. Between 2015 and 2016 59 women over the age of 18 with verified BC and signed informed consent were included in the study. Patients with stage II and III were included. CTCs were evaluated in the blood before the start of treatment, after 2 courses of chemotherapy and before surgical treatment. To detect CTCs we used the ISET (Isolation by Size of Tumor cells) technique, based on vacuum cell filtration through a membrane with a given pore size of 8 mcm. Results. In 22 (37%) women CTCs were detected before treatment, including 6 (10%) patients with CTCs found on every stage of treatment. Among 37 (63%) patients without baseline CTCs 19 (32%) developed them in the process of chemotherapy. Baseline CTCs correlated with tumor size more than 25 mm and stage III disease. Also a tendency to increased CTC prevalence in patients with poor pathological response to chemotherapy was revealed. Conclusions. CTCs represent a novel biomarker. Yet, methodology needs to be standardized and more structured studies are badly needed to reveal their true biological relevance.

作者简介

Yu Nenakhova

Russian Medical Academy of Continuing Postgraduate Education of the Ministry of Health of the Russian Federation

Email: lyullikalya@mail.ru
аспирант каф. онкологии 125993, Russian Federation, Moscow, ul. Barrikadnaia, d. 2/1

V Lyadov

Russian Medical Academy of Continuing Postgraduate Education of the Ministry of Health of the Russian Federation

канд. мед. наук, доц. каф. онкологии 125993, Russian Federation, Moscow, ul. Barrikadnaia, d. 2/1

I Poddubnaya

Russian Medical Academy of Continuing Postgraduate Education of the Ministry of Health of the Russian Federation

акад. РАН, д-р мед. наук, проф., зав. каф. онкологии 125993, Russian Federation, Moscow, ul. Barrikadnaia, d. 2/1

参考

  1. Moore G.E, Sandberg A, Schubarg J.R. Clinical and experimental observations of the occurrence and fate of tumor cells in the blood stream. Ann Surg 1957; 146:580-7.
  2. Ковалев А.А, Грудинская Т.А, Кузнецова Т.Л. и др. Гетерогенность циркулирующих опухолевых клеток. Онкология. 2012; 14 (2): 126-9.
  3. Vona G, Sabile A, Louha M et al. Isolation by size of epithelial tumor cells: a new method for the immunomorphological and molecular characterization of circulatingtumor cells. AmJPathol 2000; 156 (1): 5 "7-63
  4. Cristofanilli M, Budd G, Ellis M et al. Circulating tumor cells, disease progression, and survival in metastatic breast. Cancer Semin Oncol 2006; 33:9-14.
  5. Ненахова Ю.Н., Лядов В.К., Поддубная И.В. Циркулирующие опухолевые клетки: биология, методы выделения, клиническое значение при раке молочной железы. Современная Онкология. 2016; 18 (3): 76-82.
  6. Fehm T, Solomayer E.F, Meng S. At al. Methods for isolating circulating epithelial cells and criteria for their classification as carcinoma cells. Cytotherapy 2005; 7 (2): 171-85.
  7. Лучинская М.В. Эпителиально-мезенхимальный переход в норме и патологии. Арх. патологии. 2015; 1: 75-83.
  8. Fehm T, Solomayer E.F, Meng S. Methods for isolating circulating epithelial cells and criteria for their classification as carcinoma cells. Cytotherapy 2005; 7 (2): 171-85.
  9. Лядов В.К., Скрыпникова М.А, Попова О.П. Выделение циркулирующих в крови опухолевых клеток методом «изоляции по размеру (ISET)» (обзор). Вопр. онкологии. 2014; 60 (5): 548-52.
  10. Paterlini-Brochot P. Circulating tumor cells: who is the killer? Cancer Microenvironment2014; 7 (3): 161-76.
  11. Krebs M.G, Hou J.M, Sloane R и др. Analysis of circulating tumor cells in patients with non-small cell lung cancer using epithelial marker-dependent and - independent approaches. J Thorac Oncol 2012; 7 (2): 306-15.
  12. Farace F, Massard C, Vimond N и др. A direct comparison of Cell Search and ISET for circulating tumour-cell detection in patients with metastatic carcinomas. BrJ Cancer 2011; 105 (6): 847-53.
  13. Kallergi G, Politaki E, Alkahtani S. Cell Physiol Biochem. Evaluation of Isolation Methods for Circulating Tumor Cells (CTCs). Cell Physiol Biochem 2016; 40 (3-4): 411-9.
  14. Исмаилова Г., Laget S., Paterlini-Brechot P. Диагностика циркулирующих опухолевых клеток с помощью технологии ISET и их молекулярная характеристика для жидкостной биопсии. Clin Med Kazakhstan. 2015; 1 (35)-' 15-2 0.
  15. Mikulova V, Cabihakova M, Janatkova I. Detection of circulating tumor cells during follow-up of patients with early breast cancer: Clinical utility for monitoring of therapy efficacy. Scand J Clin Lab Invest 2014; 74 (2): 132-42.
  16. Lu Y.J, Wang P, Wang X. The significant prognostic value of circulating tumor cells in triple-negative breast cancer: a meta-analysis. Oncotarget2016; 7 (24):37361-9.
  17. Pinzani P, Salvadori B., Simi L. et al. Isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer: correlation with real-time reverse transcriptase-polymerase chain reaction results and feasibility of molecular analysis by laser microdissection. Hum Pathol 2006; 37 (6): 711-8.
  18. Gafforio J.J, Serrano M.J, Sanches Rovira P et al. Detection of breast cancer cells in the peripheral blood is positively correlated with estrogen receptor status and predicts for poor prognosis. Int J Cancer 2003; 107 (6):984-90.
  19. Smith B.M, Slade M.J, English J et al. Response of circulating tumor cells to systemic therapy in patients with metastatic breast cancer: comparison of quantative polimerase chain reaction and immunocytochemical techniques. J Clin Oncol 2000; 7:1432-9.
  20. Apostolaki S, Perraki M, Pallis A. et al. CirculatingHER2 _positive cells in the peripheral blood of patients with stage I and II breast cancer after the administration of adjuvant chemotherapy: evaluation of their clinical relevance. Ann Oncol 2007; 18 (5): 851-8.
  21. Bidard F.C, Proudhona C, Pierga J. Circulating tumor cells in breast cancer.Mol Oncol 2016; 10:418-30.
  22. Riethdorf S, Muller V, Zhang L. et al. Detection and Her2 Expression of Circulating Tumor Cells: Prospective Monitoring of Dreast Cancer Patient Treated in the Neoadjuvant GeparQuattro Trial Clin Cancer Res 2010; 16 (9): 79-93.
  23. Bidard F.C, Matiot C, Delaloge S. et al. Single circulating tumor cell detection and overall survival in nonmetastatic breast cancer. Ann Oncol. 2010; 4 (2): 729-33.

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