The analysis of the relationship between transferrin receptor 1 (TfR1) and clinical, morphological and immunophenotypic characteristics of breast cancer: retrospective cohort study
- Authors: Chulkova S.V.1,2, Sholokhova E.N.1, Poddubnaya I.V.3, Stilidi I.S.1,2, Tupitsyn N.N.1
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Affiliations:
- Blokhin National Medical Research Center of Oncology
- Pirogov Russian National Research Medical University
- Russian Medical Academy of Continuous Professional Education
- Issue: Vol 24, No 3 (2022)
- Pages: 355-360
- Section: CLINICAL ONCOLOGY
- URL: https://journals.rcsi.science/1815-1434/article/view/109435
- DOI: https://doi.org/10.26442/18151434.2022.3.201821
- ID: 109435
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Abstract
Background. Transferrin receptor 1 (TfR1) expression has been identified in a number of malignant tumors. It is noted that its overexpression gives growth advantages to cancer cells. Estimation of transferrin receptor expression in breast cancer (BC) might be an important component in disease prognosis, choice of treatment, also might be an attractive target for targeted therapy.
Aim. To evaluate the expression of TfR1 by BC cells and to study its relationship with the clinical, morphological and immunophenotypic characteristics of the tumor.
Materials and methods. This study included 82 patients with BC who received treatment at the Blokhin National Medical Research Center of Oncology (Moscow). The expression of TfR1 on primary tumor cells was studied, the relationship of TfR1 with clinical, morphological and immunophenotypic characteristics of BC was analyzed. Immunophenotyping of the primary tumor was performed by the immunohistochemical method (immunofluorescent staining) on cryostat sections. Antibodies to CD71, CD95, CD54, CD29, MUC1, Pgp170 were used. The reaction was evaluated using a luminescent microscope (AXIOSKOP, Germany). The study was dominated by patients with stage IIB – 54% and IIIB – 21%. Infiltrative ductal BC was diagnosed in 67% (n=55) of patients, infiltrative-lobular – in 22% (n=18) of cases, other types – in 11.0% (n=9).
Results. BC cells expressed TfR1 in most cases (64.4%; n=61). A combination of TfR1 monomorphic expression with MUC1 monomorphic expression (74.4%; n=47) was noted. CD29 is presented both mosaic (38.7%) and monomorphic (51.6%). The Pgp170 antigen was monomorphically observed in 27.5% of cases. As the proportion of TfR+ cells increased, the expression frequency of the adhesion molecule CD54 increased from 10.5 to 33.3%, a positive correlation was established (r=0.293; p=0.008). In the group with TfR1 monomorphic expression, the frequency of tumors expressing the CD95 apoptosis molecule decreased: 25.0% vs 13% (p=0.042).
Conclusion. BC cells overexpress TfR1. TfR1 expression is associated with tumor immunophenotype.
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##article.viewOnOriginalSite##About the authors
Svetlana V. Chulkova
Blokhin National Medical Research Center of Oncology; Pirogov Russian National Research Medical University
Author for correspondence.
Email: chulkova@mail.ru
ORCID iD: 0000-0003-4412-5019
Cand. Sci. (Med.), Assoc. Prof.
Russian Federation, Moscow; MoscowElena N. Sholokhova
Blokhin National Medical Research Center of Oncology
Email: enshell@mail.ru
ORCID iD: 0000-0002-1456-1904
Cand. Sci. (Med.)
Russian Federation, MoscowIrina V. Poddubnaya
Russian Medical Academy of Continuous Professional Education
Email: chulkova@mail.ru
ORCID iD: 0000-0002-0995-1801
SPIN-code: 1146-9889
D. Sci. (Med.), Prof., Acad. RAS
Russian Federation, MoscowIvan S. Stilidi
Blokhin National Medical Research Center of Oncology; Pirogov Russian National Research Medical University
Email: chulkova@mail.ru
ORCID iD: 0000-0002-0493-1166
D. Sci. (Med.), Prof., Acad. RAS
Russian Federation, Moscow; MoscowNikolai N. Tupitsyn
Blokhin National Medical Research Center of Oncology
Email: chulkova@mail.ru
ORCID iD: 0000-0003-3966-128X
D. Sci. (Med.), Prof.
Russian Federation, MoscowReferences
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