Stimulating effect of peptides in organotypic tissue culture of rat genitourinary system

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Abstract

Introduction. One of the actual task of modern biology and medicine is the creation of bioregulator preparation, which have the stimulating effect on the organs of genitourinary system.

Purpose of study. Study of stimulating effect on cellular proliferation of two- and three- peptides in organotypic culture of rat bud, bladder, testis, prostate, ovary.

Methods. The method was used of organotypic cultivation of genitourinary system tissues from rats. 3 ml of the nutrient medium was added in the control petry dishes. In other dishes were added the nutrient medium and the short peptides in the concentration 2 ng/ml.

Results. By the action of two- and three- peptides the square index of rat bud, bladder, testis, prostate, ovary explants was increased statistically significant, as compared to control.

Conclusion. The data obtained by the method of organotypic cultivation about stimulation of genitourinary system tissues creates the basis for the development of new preparations, for treatment of genitourinary pathology.

About the authors

Galina A. Ryzhak

Saint Petersburg Institute of Bioregulation and Gerontology

Email: galina@gerontology.ru
ORCID iD: 0000-0003-2536-1438

Director, Doctor of medical sciences, Рrofessor

Russian Federation, Ave. Dinamo 3, Saint-Petersburg, 197110

Natalia I. Chalisova

Saint Petersburg Institute of Bioregulation and Gerontology; Pavlov Institute of Physiology of the Russian Academy of Sciences

Author for correspondence.
Email: ni_chalisova@mail.ru
ORCID iD: 0000-0002-2371-0043

Expert scientific worker, Laboratory of Oncogerontology of Biogerontology Department; Leading Researcher, Laboratory of Neurogenetics, Doctor of medical sciences, Рrofessor

Russian Federation, Ave. Dinamo 3, Saint-Petersburg, 197110; nab. Makarova, 6, Saint-Petersburg, 199034

References

  1. Журкович И.К., Ковров Н.Г., Рыжак Г.А., Миронова Е.С., Хавинсон В.Х. Идентификация коротких пептидов в составе полипептидных комплексов, выделенных из органов животных. Успехи современной биологии. 2020; 2: 140–8. [Jurkovich I.K., Kovrov N.G., Rijak G.A., Mironova E.S., ., Khavinson V.Kh. Identification of short peptides in the composition of polypeptides complexes, dedicated from animals organs. Newsletter of experimental biology and medicine. 2020; 2: 140–8 [In Russian)].
  2. Хавинсон В.Х., Соловьев А.Ю., Тарновская С.И., Линькова Н.С. Механизм биологической активности коротких пептидов: проникновение в клетку и эпигенетическая регуляция экспрессии генов. Успехи современной биологии. 2013; 3: 310–6. [Khavinson V.Kh., Soloviev A.YU., Tarnovskaya S.I., Linkova N.S. Mechanism of short peptides biology activity: penetration in cell and epigenetic regulation of genes expression. Newsletter of experimental biology and medicine. 2013; 3: 310–6 [In Russian)].
  3. Khavinson V., Linkova N., Kozhevnikova E., Dyatlova A., Petukhov M. Review Transport of Biologically Active Ultrashort Peptides Using POT and LAT Carriers. International J. of Molecular Sciences. 2022: 23: 77–83.
  4. Linkova N., Khavinson V., Diatlova A., Myakisheva S., Ryzhak G. Review Peptide Regulation of Chondrogenic Stem Cell Differentiation. International J. of Molecular Sciences. 2023; 9: 84–95.
  5. Vanyushin B.F., Khavinson V.Kh., Short Biologically Active Peptides as Epigenetic Modulators of Gene Activity. Epigenetics – A Different Way of Looking at Genetics. W. Doerfler, P. Böhm (eds.). Springer International Publishing Switzerland. 2016; 69–90.
  6. Caputi S., Trubiani O., Sinjari B., Trofimova S., Diomede F., Linkova N., Diatlova A., Khavinson V. Effect of short peptides on neuronal differentiation of stem cells. International J. of Immunopathology and Pharmacology. 2019; 33: 1–12.
  7. Khavinson V.Kh., Tendler S.M., Kasyanenko N.A. Tetrapeptide KEDW Interacts with DNA and Regulates Gene Expression. Am. Journal Biomed. Sci., 2015; 7 (3): 156–69.
  8. Хавинсон В.Х. Лекарственные пептидные препараты. Прошлое, настоящее, будущее. Клиническая медицина. 2020; 98 (3): 165–77. [Khavinson V.Kh., Medicinal peptide product. Past, present, future. Clinical medicine. 2020; 98 (3): 165–77 [In Russian)].
  9. Заморский И.И., Щудрова Т.С., Линькова Н.С. и др. Пептиды восстанавливают функциональное состояние почек при цисплатиновой острой почечной недостаточности. Бюл. экспер. биол. 2015; 159 (6): 708–12. [Zamorskiy I.I., Shchudrova T.S., Linkova N.S. i dr. Peptides restore the functional state of the kidneys in cases of cisplatin-induced acute renal failure. Byul. eksper. biol. 2015; 159 (6): 708–12 [In Russian)].
  10. Заморский И.И., Щудрова Т.С., Линькова Н.С. и др. Нефропротективное действие пептида EDL при остром повреждении почек различного генеза. Бюл. эксп. биол. мед. 2017; 163 (3): 376–80. [Zamorskiy I.I.. Shchudrova T.S.. Linkova N.S. i dr. The nephroprotective effect of the EDL peptide in acute kidney damage of various origins. Byul. eksp. biol. med. 2017; 163 (3): 376–80 [In Russian)].
  11. Хавинсон В.Х., Линькова Н.С., Полякова В.О. и др. Пептиды регулируют экспрессию сигнальных молекул в клеточных культурах почек при старении in vitro. Бюл. эксп. биол. мед. 2014; 157 (2): 227–30. [Khavinson V.Kh.. Linkova N.S.. Polyakova V.O. i dr. Peptides regulate the expression of signaling molecules in kidney cell cultures during in vitro aging. Byul. eksp. biol. med. 2014; 157 (2): 227–30 [In Russian)].

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