Salivary biomarkers in adolescents with type 1 diabetes mellitus (pilot study)
- 作者: Shcherbakova M.M.1, Morozova N.S.1, Allahverdiev A.F.1, Lizunova I.Y.1, Saushkina A.A.1, Zaharova N.B.2
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隶属关系:
- Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
- Federal State Budgetary Educational Institution of Higher Education “Saratov State Medical University named after V.I.Razumovsky”, Healthcare Ministry of the Russian Federation
- 期: 卷 23, 编号 2 (2025)
- 页面: 32-37
- 栏目: Original research
- URL: https://journals.rcsi.science/1728-2918/article/view/293271
- DOI: https://doi.org/10.29296/24999490-2025-02-04
- ID: 293271
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详细
Introduction. Type 1 diabetes mellitus is a chronic autoimmune disease associated with severe complications of organs and systems, including complications of the course of dental diseases.
Material and methods. Results of 55 patients with type 1 DM became the material of laboratory-diagnostic studies. Patients with type 1 diabetes were divided into 2 groups depending on blood glucose levels: Group 1 (n=28) – patients with glucose levels ≥7 mmol/l; Group 2 (n=27) – patients with glucose levels ≤7 mmol/l; Control group – 28 patients. Periodontal tissues were evaluated by indices: PMA, SBI. OHI-S index reflected the state of oral hygiene. Mucosal immunity of oral fluid was studied using ELISA analysis of pro- and anti-inflammatory cytokines IL-8, IL-10, IL-1β, MCP-1, TNF-α, VEGF-А.
Results. The content in unstimulated oral fluid of IL-1β in patients with type 1 DM was 84.1 (95–74.6) pg/mL in the group with glucose ≤ 7 mmol/L, 80.1 (115.4–64.9) pg/mL in the group with glucose ≥7 mmol/L, 23.1 (28.2–18.6) pg/mL in the comparison group, VEGF-A was 1340 (1545–967.5) pg/mL, 1170 (1517.5–954.8) pg/mL in group 2, and 1655 (1860–1370.5) pg/mL in group 3. TNF-α was higher in group 1 – 9.8 (11.4–7.5) pg/mL than in group 2 – 8.3 (10.3–6.6) pg/mL and in group 3 – 1 (1.1–0.9) pg/mL (p < 0.05).
Conclusion. ROC curve analysis showed that these biomarkers have better sensitivity and specificity. The increase of IL-8, TNF-α, IL-1β content in mixed saliva up to 285.5 (339.8–214.5) pg/ml, 9.8 (11.4–7.5), 84.1 (95–74.6) pg/ml, respectively, was characterized by high medians of OHI-s, PMA and SBI indices, associated with high activity of inflammatory-destructive changes in periodontal tissues and impaired metabolic control of glucose level.
作者简介
Maria Shcherbakova
Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
编辑信件的主要联系方式.
Email: marya.scherbakova@yandex.ru
ORCID iD: 0009-0006-1816-7839
5th year student, Department of propaedeutics of dental diseases, E.V. Borovsky Institute of Dentistry
俄罗斯联邦, Trubetskaya str., 8, build. 2, Moscow, 119991Natalia Morozova
Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
Email: morozova_n_s_2@staff.sechenov.ru
ORCID iD: 0000-0002-6453-1615
SPIN 代码: 4654-9842
Doctor of Medical Sciences, Professor of the Department of Propaedeutics of the Department of Propaedeutics of Stomatologic Diseases, E.V. Borovsky Institute of Dentistry
俄罗斯联邦, Trubetskaya str., 8, build. 2, Moscow, 119991Arif Allahverdiev
Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
Email: arif_17_03_1998@mail.ru
ORCID iD: 0009-0000-9209-3803
Department Applicant of Propaedeutics of Dental Diseases, E.V. Borovsky Institute of Dentistry
俄罗斯联邦, Trubetskaya str., 8, build. 2, Moscow, 119991Irina Lizunova
Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
Email: i.lizun0va@yandex.ru
ORCID iD: 0009-0008-2470-8667
5th year student
俄罗斯联邦, Trubetskaya str., 8, build. 2, Moscow, 119991Alina Saushkina
Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
Email: saushkina.alina@mail.ru
ORCID iD: 0009-0007-4654-6905
5th year student
俄罗斯联邦, Trubetskaya str., 8, build. 2, Moscow, 119991Natalia Zaharova
Federal State Budgetary Educational Institution of Higher Education “Saratov State Medical University named after V.I.Razumovsky”, Healthcare Ministry of the Russian Federation
Email: lipidgormon@mail.ru
ORCID iD: 0000-0001-9410-2240
SPIN 代码: 5354-6327
Doctor of Medical Sciences, Professor, Department of Biochemistry and Clinical Laboratory Diagnostics
俄罗斯联邦, Bol'shaya Kazach'ya str., 112, Saratov, 410012参考
- Диабет. Всемирная организация здравоохранения. 16.09.2022. «Доступно на» https://www.who.int/ru/news-room/fact-sheets/detail/diabetes [дата обращения: 18.10.2024] [Diabetes. World Health Organization. 09/16/2022. «Available at» https://www. who.int/ru/news-room/fact-sheets/detail/diabetes [date of application: 10/18/2024] (In Russian)].
- Митронин А.В., Антонова О.А. Биомаркеры смешанной слюны как индикаторы состояния организма. Российская стоматология. 2022; 15 (1): 61–2. doi: 10.17116/rosstomat20221501125. [Mitronin A.V., Antonova O.A. Biomarkers of mixed saliva as indicators of the state of the organism. Russian dentistry. 2022; 15 (1): 61–2. doi: 10.17116/rosstomat20221501125 (In Russian)].
- Faura G., Boix-Lemonche G., Holmeide A. R., Verkauskiene R., Volke V., Sokolovska J., Petrovski G. Colorimetric and Electrochemical Screening for Early Detection of Diabetes Mellitus and Diabetic Retinopathy-Application of Sensor Arrays and Machine learning. Sensors (Basel, Switzerland). 2022; 22 (3): 718. doi: 10.3390/s22030718.
- Bolyarova-Konova T., Petkova S., Mihaylova H., Velikova T., Ivanova-Todorova E., Tumangelova-Yuzeir K., Todorova D. Concentrations of interleukin-1ß in gingival crevicular fluid and saliva – a potential diagnostic biomarker of periodontal diseases. – Folia Med (Plovdiv). 2020; 62 (4): 825–30. doi: 10.3897/folmed.62.e49872
- Chakraborty P., Mukhopadhyay Pю, Bhattacharjee K., Chakraborty A., Chowdhury S., Ghosh S. Periodontal Disease in Type 1 Diabetes Mellitus: Influence of Pubertal Stage and Glycemic Control. Endocr Pract. 2021; 27 (8): 765–8. doi: 10.1016/j.eprac.2021.01.010.
- Ferizi L., Bimbashi V., Kelmendi J. Association between metabolic control and oral health in children with type 1 diabetes mellitus. BMC Oral Health. 2022; 22 (1): 502. doi: 10.1186/s12903-022-02555-x.
- Bui F.Q., Almeida-da-Silva C.L.C., Huynh B. et al. Association between periodontal pathogens and systemic disease. Biomed J. 2019; 42 (1): 27–35. doi: 10.1016/j.bj.2018.12.001.
- Sanz M., Ceriello A., Buysschaert M., Chapple I., Demmer R.T., Graziani F., Herrera D., Jepsen S., Lione L. et al. Scientific evidence on the links between periodontal diseases and diabetes: Consensus report and guidelines of the joint workshop on periodontal diseases and diabetes by the International Diabetes Federation and the European Federation of Periodontology. J. Clin. Periodontol. 2018; 45 (2): 138–49. doi: 10.1111/jcpe.12808.
- Доменюк Д.А., Давыдов Б.Н., Гильмиярова Ф.Н., Ивченко Л.Г. Клинико-диагностическое значение активности матриксных металлопротеиназ и их тканевых ингибиторов в оценке состояния тканей пародонта у детей с сахарным диабетом первого типа. Часть II, Стоматология детского возраста и профилактика. 2018; 17, 1 (64): 37–46. doi: 10.25636/PMP.3.2018.1.9. EDN XOTKPZ. [Domenyuk D.A., Davydov B.N., Gilmiyarova F.N., Ivchenko L.G. Clinical and diagnostic value of matrix metalloproteinases activity and their tissue inhibitors in the assessment of periodontal tissue condition in children with type I diabetes mellitus. Part II, Pediatric Dentistry and Prevention. 2018; 17, 1 (64): 37–46. doi: 10.25636/PMP.3.2018.1.9. EDN XOTKPZ (in Russian)]
- Ушницкий И.Д., Иванова А.А., Пинелис И.С., Юркевич А.В., Михальченко Д.В. Современные этиологические и патогенетические аспекты воспалительно – деструктивных процессов тканей пародонта. Эндодонтия today. 2019; 17 (4): 46–9. doi: 10.36377/1683-2981-2019-17-4-46-49. [Ushnitsky I.D., Ivanova A.A., Pinelis I.S., Yurkevich A.V., Mikhalchenko D.V. Modern etiologic and pathogenetic aspects of inflammatory and destructive processes of periodontal tissues. Endodontia Today. 2019; 17 (4): 46–9. doi: 10.36377/1683-2981-2019-17-4-46-49 (in Russian)]
- Камалова Ф.Р. Клинико-иммунологическая оценка полости рта у детей с сахарным диабетом 1-типа. Астана медициналық журналы. 2020. «Доступно на» file:///C:/Users/mary_/Downloads/kliniko-immunologicheskaya-otsenka-polosti-rta-u-detey-s-saharnym-diabetom-1-tipa%20(1).pdf. [дата обращения: 18.10.2024] [Kamalova F.R. Clinical and immunological assessment of the oral cavity in children with type 1 diabetes mellitus. Astana meditsinalyk J. 2020. «Available at» file:///C:/Users/mary_/Downloads/kliniko-immunologicheskaya-otsenka-polosti-rta-u-detey-s-saharnym-diabetom-1-tipa%20(1).pdf. [date of access: 10/18/2024] (In Russian)]
- Даминова Ш., Махсумова C., Махсумова И., Ахматова З. Оценка состояния гуморального и местного иммунитета полости рта у детей больных сахарным диабетом типа 1. Стоматология. 2023; 1 (4): 62–5. «Доступно на» https://inlibrary.uz/index.php/stomatologiya/article/view/21701 [дата обращения: 18.10.2024] [Daminova Sh., Makhsumova C., Makhsumova, I., & Akhmatova, Z. Assessment of the state of humoral and local immunity of the oral cavity in children with type 1 diabetes mellitus. Dentistry. 2023; 1 (4): 62–65 (In Russian)]
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