Analysis of the composition of microbiota of the oral mucosa and recurrent aphthae
- Authors: Akmalova G.M.1, Galeev R.V.2, Gimranova I.A.1, Mannapova G.R.1
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Affiliations:
- Bashkir State Medical University
- Children’s Dental Clinic No. 7, Ufa
- Issue: Vol 28, No 1 (2024)
- Pages: 87-92
- Section: Clinical Investigation
- URL: https://journals.rcsi.science/1728-2802/article/view/264183
- DOI: https://doi.org/10.17816/dent631690
- ID: 264183
Cite item
Abstract
BACKGROUND: Recurrent aphthous stomatitis is a common disease of the oral mucosa. One of the most substantiated theories of the occurrence of recurrent aphthous stomatitis is the role of immunobacterial mechanisms.
AIM: To study the composition of the microbiota of the oral mucosa and recurrent aphthae in children.
MATERIALS AND METHODS: Thirty-five children with aphthous stomatitis aged 7–14 years were examined. The patients underwent a microbiological study of the microbiota from the lesion (aphtha) and unaffected oral mucosa.
RESULTS: Fifteen types of microorganisms, represented by aerobes and anaerobes, were identified. Streptococcus significantly enriches the microbiota of the oral mucosa, and Streptococcus, E. сoli, and S. epidermidis significantly enrich the microbiota of the aphthae. Additionally, the number of yeast-like cells of the genus Candida in the lesions significantly increased (7.13±2.68 lg CFU/ml). In children, the surface of the aphthae had increased S. mutans (5.47±1.83 lg CFU/ml) compared to the oral mucosa, where the number was 2.35±0.12 lg CFU/ml, with an incidence of 93 and 100%, respectively. S. sanguinis was found equally in both groups (100%), the amount of infestation of which did not differ significantly. S. aureus and Klebsiella spp. were found in 93 and 18% of cases, respectively, only in aphthae, and the number was significantly high (p <0.001) at 5.13±1.68 and 4.13±1.38 lg CFU/ml, respectively, than on oral mucosa.
CONCLUSION: A change in the microflora in the oral cavity was established in all children with recurrent aphthous stomatitis — a decrease in the lesions (aphthae) in the representatives of Streptococcus in bacterial communities, Lactobacillus with an increase in the number of yeast-like cells of the genus Candida and the appearance in the biocenosis from the surface of aphthous ulcers S. aureus, E. coli and S. epidermidis. Streptococcus significantly prevails in the oral mucosa, and Streptococcus, E. coli, and S. epidermidis colonize the aphthae.
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##article.viewOnOriginalSite##About the authors
Guzel M. Akmalova
Bashkir State Medical University
Author for correspondence.
Email: Akmalova-ekb@yandex.ru
ORCID iD: 0000-0001-7745-0489
SPIN-code: 2972-2346
Russian Federation, Ufa
Ruslan V. Galeev
Children’s Dental Clinic No. 7, Ufa
Email: dent-imp@mail.ru
ORCID iD: 0009-0006-5626-9414
Russian Federation, Ufa
Irina A. Gimranova
Bashkir State Medical University
Email: mia8408@mail.ru
ORCID iD: 0000-0003-3330-9437
Russian Federation, Ufa
Guzel R. Mannapova
Bashkir State Medical University
Email: mannapova.81@mail.ru
ORCID iD: 0000-0001-9927-1203
Russian Federation, Ufa
References
- Yang Z, Cui Q, An R, et al. Comparison of microbiomes in ulcerative and normal mucosa of recurrent aphthous stomatitis (RAS)-affected patients. BMC Oral Health. 2020;20(1):128. doi: 10.1186/s12903-020-01115-5
- Rabinovich OF, Abramova ES, Umarova KV, Rabinovich IM. Aetiology and pathogenesis of recurrent ulcerative stomatitis. Clinical Dentistry (Russia). 2015;(4):8–13. EDN: VEBNNR
- Chavan M, Jain H, Diwan N, et al. Recurrent aphthous stomatitis: a review. J Oral Pathol Med. 2012;41(8):577–583. doi: 10.1111/j.1600-0714.2012.01134.x
- Légeret C, Furlano R. Oral ulcers in children — a clinical narrative overview. Ital J Pediatr. 2021;47(1):144. doi: 10.1186/s13052-021-01097-2
- Koberová R, Merglová V, Radochová V. Recurrent aphthous stomatitis in children: a practical guideline for paediatric practitioners. Acta Medica (Hradec Kralove). 2020;63(4):145–149. doi: 10.14712/18059694.2020.56
- Soldatov IK, Juravleva LN, Tegza NV. Scientometric analysis of dissertation papers on pediatric dentistry in Russia. Russian Journal of Dentistry. 2023;27(6):571–580. EDN: QINWXB doi: 10.17816/dent624942
- Davydova MM, Ippolitov EV, Nikolaeva EN, et al. Microbiology, virology and immunology of the oral cavity. Tsarev VN, editor. Moscow: GJeOTAR-Media; 2019. 720 p. (In Russ). EDN: KUPELM doi: 10.33029/9704-5055-0-MVI-2019-1-720
- Makedonova YuA, Venskel EV, Alexandrina ES, Kalashnikova SA. Anatomical changes in the oral mucosa in aphthous stomatitis. Vestnik nauchnyh konferencij. 2023;(2-3):75–77. EDN: PGHNLA
- Kim YJ, Choi YS, Baek KJ, et al. Mucosal and salivary microbiota associated with recurrent aphthous stomatitis. BMC Microbiol. 2016;16 Suppl. 1:57. doi: 10.1186/s12866-016-0673-z