Changes in the enamel surface in patients with polymorphism of the VDR gene under the action of remineralizing composition and vitamin D
- Authors: Tikhonova S.N.1, Kozlova M.V.1, Gorbatova E.A.1, Evstafyeva O.L.2
-
Affiliations:
- Central State Medical Academy at the Department of Presidential Affairs of the Russian Federation
- Russian University of Medicine
- Issue: Vol 28, No 1 (2024)
- Pages: 61-69
- Section: Clinical Investigation
- URL: https://journals.rcsi.science/1728-2802/article/view/264180
- DOI: https://doi.org/10.17816/dent623808
- ID: 264180
Cite item
Abstract
BACKGROUND: The leading role in the pathogenetic mechanism of caries development is the imbalance between the de and remineralization of hard tooth tissues. The combination of VDR alleles affects calcium-phosphate metabolism. In 65% of the human population, the structure of the VDR gene has a genetic polymorphism.
AIM: To study the effect of vitamin D and a remineralizing paste on the enamel surface structure in patients with genetic polymorphism of VDR gene receptors.
MATERIALS AND METHODS: In 18 individuals with a polymorphism index of the VDR A/A gene, a laboratory indicator of 25 (OH) vitamin D in the blood of 18.20±1.84 ng/ml, according to orthodontic indications, the retinated third molars in the lower jaw were removed. Then, the samples were lowered into three flasks of six pieces with 100 ml of artificial saliva. Containers with teeth were divided into groups: group 1, with a solution of artificial saliva only; group 2, an additional 1,000 IU of an aqueous solution of vitamin D; and group 3, tooth enamel was treated for 2 minutes with an electric toothbrush with a paste containing a remineralizing composition, immersed in artificial saliva with cholecalciferol 1,000 IU. The flasks were in the thermostat at a temperature of 37.4 C per day. In all samples, electron microscopy of the enamel contact surface at the level of the crown equator was performed using a Tescan Mira 3 LMU microscope (TESCAN, Czech Republic) with an Oxford X-MAX 5 energy dispersive X-ray detector (Oxford Instruments, United Kingdom) with analysis of local mineral composition.
RESULTS: In group 1, with electron microscopy of enamel, areas of increased mineralization alternated with areas of reduced density. The coefficient of the molar ratio Ca/P was 1.32±0.13, indicating the destruction of hydroxyapatites. In group 2 the intercrystalline pore space was filled with calcium and phosphate ions. In group 3, the enamel surface acquired a smooth homogeneous relief. An increase in the weight percentage of trace elements was observed, and the Ca/P ratio was 2.20±0.02, which indicated a phase of remineralization.
CONCLUSION: Patients with VDR gene polymorphism have a vitamin D deficiency in the blood serum. The trace element composition of the enamel surface is characterized by a phase of demineralization. Treatment with remineralizing paste and addition of cholecalciferol solution to artificial saliva changes the structure of hydroxyapatite and increases the caries-resistant properties of enamel.
Full Text
##article.viewOnOriginalSite##About the authors
Svetlana N. Tikhonova
Central State Medical Academy at the Department of Presidential Affairs of the Russian Federation
Author for correspondence.
Email: tixonovalana@gmail.com
ORCID iD: 0009-0004-7372-8833
SPIN-code: 6610-7509
Russian Federation, Moscow
Marina V. Kozlova
Central State Medical Academy at the Department of Presidential Affairs of the Russian Federation
Email: profkoz@mail.ru
ORCID iD: 0000-0002-3066-206X
SPIN-code: 5546-2489
MD, Dr. Sci. (Medicine), Professor
Russian Federation, MoscowEkaterina A. Gorbatova
Central State Medical Academy at the Department of Presidential Affairs of the Russian Federation
Email: gorbatova_k@mail.ru
ORCID iD: 0000-0002-7729-7979
SPIN-code: 5836-2399
MD, Cand. Sci. (Medicine), Associate Professor
Russian Federation, MoscowOksana L. Evstafyeva
Russian University of Medicine
Email: evstafieva_ol@mail.ru
ORCID iD: 0000-0003-2358-0557
SPIN-code: 5455-8875
MD, Cand. Sci. (Medicine), Associate Professor
Russian Federation, MoscowReferences
- Fedorova RK. Comparative analysis of stomatologic morbidity and hygienic state of the mouth in students of 3 and 5 courses of the Institute of Stomatology. In: Molodezh — Barnaul: Proceedings of the XXIV city scientific-practical conference of young scientists. Barnaul: Altai State University; 2022. P. 687–689. (In Russ). EDN: ZAIEUA
- Filippenko NA. Research of ways of treatment of dental caries and its complications. In: Science of young people — the future of Russia: collection of scientific articles of the 6th International Scientific Conference of promising developments of young scientists. Volume 3. Kursk: South-West State University; 2021. P. 313–316. (In Russ). EDN: ZAKEGD
- Shevchenko OV. The impact of primary prevention programs of dental diseases on the main trends in the development of dental services. Stomatology. 2023;102(2):91–96. EDN: TYDKKA doi: 10.17116/stomat202310202191
- Chernyavskaya ND, Terekhova TN. Detection of initial occlusive caries in the first permanent molars by laser fluorescence and ICDAS. Sovremennaya stomatologiya. 2021;(2):26–30. EDN: VLSTKF
- Fedorov PP, Mayakova MN, Gainutdinov RV, et al. Investigation of the deposition of calcium fluoride nanoparticles on the chips of CAF2 single crystals. Condensed Matter and Interphases. 2021;23(4):607–613. EDN: KKMNKR doi: 10.17308/kcmf.2021.23/3681
- Iordanishvili AK, Orlov AK, Safronov SV, Smolnyakova AV. Morphological structure of hard dental tissues with sensitive dentin in adults of different age. Kursk Scientific and Practical Bulletin Man and His Health. 2020;(1):21–28. EDN: KDYRYU doi: 10.21626/vestnik/2020-1/03
- Bumbu BA, Luca MM, Buzatu R. Examining the role of vitamin d in caries susceptibility in children’s deciduous teeth: a systematic review. Nutrients. 2023;15(22):4826. doi: 10.3390/nu15224826
- Carlberg C, Raczyk M, Zawrotna N. Vitamin D: a master example of nutrigenomics. Redox Biol. 2023;62:102695. doi: 10.1016/j.redox.2023.102695
- Hujoel PP, Lingström P. Nutrition, dental caries and periodontal disease:a narrative review. J Clin Periodontol. 2017;44 Suppl 18:79–84. doi: 10.1111/jcpe.12672
- Sadeghi M, Golshah A, Godiny M, et al. The most common vitamin D receptor polymorphisms (ApaI, FokI, TaqI, BsmI, and BglI) in children with dental caries: a systematic review and meta-analysis. Children (Basel). 2021;8(4):302. doi: 10.3390/children8040302
- Protas VV, Poghosyan GP, Li KG, Danilenko MP. Vitamin D receptor gene polymorphisms characteristic. Bulletin of the Karaganda University. Biology. Medicine. Geography series. 2021;104(4):60–70. EDN: QBBVAV doi: 10.31489/2021BMG4/60-70
- Marozik P, Rudenka A, Kobets K, Rudenka E. Vitamin D status, bone mineral density, and VDR gene polymorphism in a cohort of belarusian postmenopausal women. Nutrients. 2021;13(3):837. doi: 10.3390/nu13030837
- Dedov II, Melnichenko GA. Endocrinology. National guide. Moscow: GEOTAR Media; 2024. (In Russ).
- Vavilova TP, Medvedev AE. Biological chemistry. Oral biochemistry: textbook. Moscow: GEOTAR Media; 2023. (In Russ).