Email this article IMMUNE ANTIBODIES IN MOKSHA AND ERZYA ETHNIC GROUPS OF THE REPUBLIC OF MORDOVIA
- Authors: Gusachenko L.A.1, Litovchenko O.G.2
-
Affiliations:
- Surgut State University
- Federal State Autonomous Educational Instition of Higher Education «South Ural State University (National research universsity)
- Issue: Vol 27, No 6 (2020)
- Pages: 22-28
- Section: Articles
- URL: https://journals.rcsi.science/1728-0869/article/view/42409
- DOI: https://doi.org/10.33396/1728-0869-2020-6-22-28
- ID: 42409
Cite item
Full Text
Abstract
ABO system's immune antibodies are common, but not as persistent as natural anti-A and anti-B antibodies. They are the result of hyper immunization of an iso- or heteroimmune nature and belong to the IgG class. The study of serum for the presence of immune antibodies of the ABO, Rh system is necessary for the diagnosis of hemolytic disease cases of newborns with ABO, Rh-conflict pregnancy. Clinically significant immune antibodies of erythrocyte systems capable to cause in vivo destruction of red blood cells are of great importance in transfusiology and immunohematology. The most common cause of their occurrence is alloimmunization with red blood cell antigens during childbirth. The aim of this study was to determine the characteristics of the immune anti-erythrocyte antibodies in residents of the Republic of Mordovia belonging to the ethnic groups of Moksha and Erzya. Methods. The cross-sectional study involved 294 people from the Moksha and 387 people and Erzya ethnic groups, randomly selected in 7 districts of the Republic of Mordovia. Detection of IgG anti-A, anti-B ABO systems was performed by inactivation of disulfide bonds in IgM molecules using a 5 % unitiol solution and then by direct agglutination method at indoor temperature with standard red blood cells of group O(I), A(II), and B(III). The study of incomplete anti-erythrocyte antibodies of other systems was performed by indirect Coombs' test using gel technology. Results. The study of immune antibodies of the ABO system revealed from 10.61 to 16.00 % of anti-A antibodies and from 2.27 to 6.38 % of anti-B antibodies in both men and women Moksha and Erzya. We studied immune anti-erythrocyte antibodies: anti-D 0.34 % in Moksha and anti-D 0.26% and anti-Kell 0.26 % antibodies in Erzya. The scale of immunogenicity of red blood cell antigens in the studied groups: A>, B>, D> and K>. The alloimmunization index of transfusion-hazardous antigens was calculated as 0.34 for Moksha and 0.52 for Erzya. Conclusions. Physiologically, the immune system in Moksha and Erzya actively reacts with antibody's production and it is sensitive to immunization by the group of antigens.
Full Text
##article.viewOnOriginalSite##About the authors
L. A. Gusachenko
Surgut State University
Email: LA264648@mail.ru
аспирант кафедры физиологии медицинского института Surgut, Russia
O. G. Litovchenko
Federal State Autonomous Educational Instition of Higher Education «South Ural State University (National research universsity)биолог лаборатории иммунологических исследований КУ
References
- Агаджанян Н. А., Макарова И. И. Этнический аспект адаптационной физиологии и заболеваемости населения // Экология человека. 2014. № 3. С. 3-13.
- Буркитбаев Ж. К., Меирбекова М. Б., Курганская Е. Н., Карп Л. Л., Магзумова Р. З. Фенотипы антигенов эритроцитов и индекс сенсибилизации у доноров компонентов крови и пациентов г. Астаны // Трансфузиология. 2013. № 2. С. 20-26.
- Гудкова Л. К. Популяционная физиология человека: традиционные подходы и новые возможности // Археология, этнография и антропология Евразии. 2009. № 2 (38). С. 144-152.
- Гусаченко Л. А., Чемакин Ю. А,. Литовченко О. Г. Антиэритроцитарные антитела доноров г. Сургута // Вестник СурГУ. 2018. № 4 (22). С. 63-67.
- Донсков С. И., Мороков В. А. Группы крови человека: руководство по иммуносерологии. М.: ИП Скороходов В. А., 2011. 1016 с.
- Донсков С. И., Уртаев Б. М., Дубинкин И. В. Новая тактика гемотрансфузионной терапии - от совместимости к идентичности: руководство для специалистов производственной и клинической трансфузиологии. М.: БИНОМ, 2015. 270 с.
- Косяков П. Н. Изоантигены и изоантитела человека в норме и патологии. М.: Медицина, 1974. 360 с.
- Липатова И. С. Аллоиммунизация групповыми антигенами эритроцитов (индивидуальные и популяционные особенности): автореф. дис.. канд. мед. наук. Москва, 2009. 26 с.
- Меркулова Н. Н. Распространенность, физиологические и иммуносерологические особенности естественных и иммунных групповых антител системы АВО у жителей Среднего Приобья: автореф. дис.. канд. биол. наук. Москва, Тюмень, 1999. 35 с.
- Рагимов А. А. Трансфузиология: национальное руководство / под ред. Рагимова А. А. М.: ГЭОТАР-Медиа, 2018. 1 104 с.
- Реброва О. Ю. Статистический анализ медицинских данных Применение пакета прикладных программ STATISTICA. М.: Медиасфера, 2006. 312 с.
- Хромова Е. А. Иммуносерологические особенности крови аборигенов Среднего Приобья: автореф. дис.. канд. биол. наук. Тюмень, 2003. 22 с.
- Хромова Е. А. Предупреждение аллоиммунизации антигенами эритроцитов у онкологических больных // Современная медицина: актуальные вопросы: сб. ст. по матер. XLIV-XLV междунар. науч.-практ. конф.: Новосибирск: СибАК, 2015. № 6-7 (41). С. 35-42.
- Boes M. Role of natural and immune IgM antibodies in immune responses // Mol. Immunol. 2000. N 37. P 1141-1149.
- Chowdhury S., Bandyopadhyay S., Mandal C., Chandra S., MandalC. Flow-cytometric monitoring of disease-associated expression of 9-O-acetylated sialoglycoproteins in combination with known CD antigens, as an index for MRD in children with acute lymphoblastic leukaemia: a two-year longitudinal follow-up study // BMC Cancer. 2008. N 10 (6). P. 539-549.
- Golovkina L. I., Stremoukhova A., Vasilieva M., Pushkina T., Atroscchenko G., Kalandarov R., Khasigova B., Surin V., Pshenichnikova O., Salomashkina V., Parovichnikova E. ABO*A and RHD Variants in Russians // Vox Sanguinis. 2017. Vol. 112, N 1. P 218.
- Hodgkin P. D. Role of cross-reactivity in the development of antibody responses // The Immunologist. 1998. Vol. 6, N 6. P 223-226.
- Janeway C. A., Travers P., Walport M. and Shlomchik M. J. Immunobiology: The Immune System in Health and Disease // Immunobiology. 5th edn., Garland Science, New York, 2001. P 884.
- Kaveri S. V., Silverman G. J., Bayry J. J. Natural IgM in Immune Equilibrium and Harnessing Their Therapeutic Potential // Immunology. 2012. N 188 (3). P. 939-945.
- Mouthon L., Haury M., Lacroix-Desmazes S., Barreau C., Coutinho A., and Kazatchkine M. D. Analysis of the normal human IgG antibody repertoire. Evidence that IgG autoantibodies of healthy adults recognize a limited and conserved set of protein antigens in homologous tissues // J. Immunol. 1995. N 154 (11). P. 5769-5778.
Supplementary files
