Effect of subchronic intoxication with thyram on the activity of antioxidant enzymes and the state of lipoperoxidation processes

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Abstract

AIM: This study aimed to investigate the effect of oxidative stress on the antioxidant defense system and state of lipid peroxidation of the body.

MATERIAL AND METHODS: Modeling of oxidative stress was carried out on 240 rats by daily administration of thiram at a dose of 1/50 LD50 (8 mg/kg body weight) in the diet of rats for 4 weeks. The catalytic activity of superoxide dismutase and catalase and the concentration of malondialdehyde and diene conjugates were analyzed.

RESULTS: The modeling of subchronic intoxication significantly decreased the activity of catalase and superoxide dismutase (p <0.05) and increased the concentration of malondialdehyde, diene conjugates in blood plasma, erythrocyte mass, and liver homogenate. After experimental subchronic intoxication, antioxidants such as vitamin E at a dose of 8.58 mg/kg and milk thistle extract at a dose of 13.74 mg/kg were used. Using such antioxidants within 30 days had significantly restored the activity of superoxide dismutase and catalase, and it also reduced the concentration of malondialdehyde and diene conjugates in all investigated body media.

CONCLUSION: Fungicide intoxication with thiram affects redox homeostasis. In addition, the usage of vitamin E and milk thistle extract can restore the prooxidant–antioxidant balance of the body.

About the authors

Ivan V. Korolev

Kursk State Medical University

Email: korolevva@kursksmu.net
ORCID iD: 0000-0002-6335-4311

medical university student
Russian Federation, Kursk

Anastasia V. Sedykh

Kursk State Medical University

Email: turquoise95@mail.ru
ORCID iD: 0000-0002-6117-0666
SPIN-code: 3374-3900

researcher
Russian Federation, Kursk

Vladimir A. Korolev

Kursk State Medical University

Email: medecol1@yandex.ru
ORCID iD: 0000-0002-4376-4284
SPIN-code: 1180-1442

Dr. Sci. (Biol.), Professor
Russian Federation, Kursk

Elena V. Felker

Kursk State Medical University

Email: felkerev@kursksmu.net
ORCID iD: 0000-0002-7948-7290
SPIN-code: 7168-7321

MD, PhD

Russian Federation, Kursk

Olga A. Medvedevа

Kursk State Medical University

Email: medvedevaoa@kursksmu.net
ORCID iD: 0000-0002-2889-155X
SPIN-code: 4394-4097

Dr. Sci. (Biol.)

Russian Federation, Kursk

Vera A. Ryadnova

Kursk State Medical University

Email: veraan@ya.ru
SPIN-code: 5629-4557

reseacher

Russian Federation, Kursk

Egor V. Korolev

Kursk State Medical University

Author for correspondence.
Email: korolevva@kursksmu.net
ORCID iD: 0000-0003-3324-8689

MD, medical university student

Russian Federation, Kursk

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Copyright (c) 2022 Korolev I.V., Sedykh A.V., Korolev V.A., Felker E.V., Medvedevа O.A., Ryadnova V.A., Korolev E.V.

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