CONDITIONING OF PHOTOINDUCED BACTERICIDAL ACTIVITY ON SURFACE OF TITANIUM DIOXIDE FILMS


Cite item

Full Text

Abstract

Special surfaces based on titanium dioxide are developed for prevention of nosocomial infections. The reactive oxygen species (ROS) have been generated by the surfaces after their UV-irradiation. ROS caused destruction of bacteria. However, effectiveness of bactericidal activity of ТiO 2-films depends on several factors. Influence of time of UV irradiation, thermal (annealing) and chemical (benzene) treatment of films on the bactericidal activity, as well as the bactericidal effect against suspended and lyophilized bacteria have been studied. Three clinical isolates Staphylococcus aureus 956, Staphylococcus epidermidis 1061, Escherichia coli 321-5 have been used. Viability of the suspended bacteria has been determined with use of the classical method of counting CFU, viability of lyophilized bacteria has been determined with use of the method of estimation of coefficients of bactericidal extinction of control and test samples. It has been shown that the bactericidal effect of thin films against all strains increased with an increase in the flow of UV exposure. dermal and chemical treatment caused bactericidal activity recovery which was lost during the initial incubation of the bacterial suspension on the surface. Absence of fluid in the system increased the bactericidal activity of TiO 2-films.

About the authors

S N Pleskova

R. E. Alekseev Nizhny Novgorod State Technical University

Email: pleskova@mail.ru
доктор биологических наук, профессор кафедры биотехнологии, физической и аналитической химии

I S Golubeva

R. E. Alekseev Nizhny Novgorod State Technical University

Yu K Verevkin

Institute of Applied Physics of Russian Academy of Sciences, Nizhny Novgorod

References

  1. Ванько Л. В., Сафронова В. Г., Матвеева Н. К., Сухих Г. Т. Оксидативный стресс в генезе акушерских осложнений. М. : ГЭОТАР Медиа, 2010. 264 с.
  2. Гланц С. Медико-биологическая статистика / пер. с англ. М. : Изд. дом «Практика», 1999. 462 с.
  3. Гржибовский А. М. Типы данных, проверка распределения и описательная статистика // Экология человека. 2008. № 1. С. 52-58.
  4. Плескова С. Н., Голубева И. С., Верёвкин Ю. К., Першин Е. А., Буренина В. Н., Королихин В. В. Фотоиндуцированная бактерицидная активность ТiO2-пленок // Прикладная биохимия и микробиология. 2011. Т. 47, № 1. С. 28-32.
  5. Семина Н. А., Прямухина Н. С., Жилина Н. Я. Заболеваемость внутрибольничными инфекциями в Российской Федерации // Журнал микробиологии, эпидемиологии и иммунобиологии. 1995. № 2. С. 30-34.
  6. Суйковская Н. В. Химические методы получения тонких прозрачных пленок. М. : Химия, 1971. 198 с.
  7. Хороших В. М., Белоус В. А. Пленки диоксида титана для фотокатализа и медицины // ФИП PSE. 2009. Т. 7, № 3. С. 223-238.
  8. Cheng Y. W. Chan R. C., Wong P. K // Water Research. 2007. Vol. 41, N 4. F! 842-852.
  9. Dancer S. J. Importance of the environment in meticillin-resistant Staphylococcus aureus acquisition: the case for hospital cleaning // Lancet Infectious Diseases. 2008. Vol. 8, N 2. Р 101-113.
  10. Friberg B., Friberg S., Burman L. G. Correlation between surface and air counts of particles carrying aerobic bacteria in operating rooms with turbulent ventilation: an experimental study // J. Hosp. Infect. 1999. Vol. 42, N 1. Р 61-68.
  11. Kochkodan V., Tsarenko S., et al. Adhesion of microorganisms to polymer membranes: a photobactericidal effect of surface treatment with Ті02 // Desalination. 2008. Vol. 220, N 1-3. P 380-385.
  12. Koizumi Y., Taya M. Photocatalytic inactivation rate of phage ms2 in titanium dioxide suspensions containing various ionic species // Biotechnology Letters. 2002. Vol. 24, N 6. Р 459-462.

Copyright (c) 2013 Ekologiya cheloveka (Human Ecology)


 


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies