GENE POLYMORPHISMS, MERCURY CONCENTRATION, AND CYTOGENETIC DAMAGE IN THE INHABITANTS OF THE MERCURY ORE MININING AND RECYCLING AREA


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Abstract

The aim of the study was to evaluate the association between the frequency of cytogenetically damaged lymphocytes, glutathione- S-transferase (GST) gene polymorphisms, and mercury concentrations in the peripheral blood of mercury industry workers as well as an non-exposed local population (control group) of Aktash village, the Republic of Altay. The venous blood samples were obtained from 184 workers and 156 controls for chromosome assay, mercury blood concentration analysis, and the GSTM1 and GSTT1 gene polymorphism assessment by a polymerase chain reaction approach. Statistical analyses were carried out using Student's t-test, one-way ANOVA, or linear multiple regression. We demonstrated a significant increase in the frequency of lymphocytes with aneuploidy and polyploidy in the workers as compared with the controls (p < 0.001). The highest levels of aneuploidal and polyploidal lymphocytes were determined in the subgroup of workers with double null GSTM1(0)/GSTT1(0) genotype (p<0.01 or p < 0.05). The study revealed significant relationship between the levels of hypoploidal and polyploidal lymphocytes according to GSTM1 or GSTT1 genotypes and mercury concentrations (p<0.001). In addition, we found no difference in the levels of chromatid and chromosome aberrations according to GSTM1 or GSTT1 genotypes (p > 0.05).

About the authors

N N Ilyinskikh

Federal State Autonomous Educational Institution of Higher Education "National Research Tomsk State University" of the Ministry of Education and Science of the Russian Federation

Email: nauka-tomsk@yandex.ru
доктор биологических наук, профессор кафедры экологического менеджмента 634050, г. Томск, а/я 808

E N Ilyinskikh

Federal State Autonomous Educational Institution of Higher Education "National Research Tomsk Polytechnic University" of the Ministry of Education and Science of the Russian Federation

I N Ilyinskikh

State Budgetary Educational Institution of Higher Professional Education "Siberian State Medical University" of the Ministry of Healthcare of the Russian Federation

Томск

O V Gvozdareva

State Budgetary Educational Institution of Higher Professional Education "Siberian State Medical University" of the Ministry of Healthcare of the Russian Federation

Томск

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