NEUTROPENIA'S FORMATION AS A CONSEQUENCE OF NEUTROPHIL MIGRATION ACTIVATION IN ALMOST HEALTHY PEOPLE IN THE NORTH


Cite item

Full Text

Abstract

The aim: to establish the causes of neutropenia formation in almost healthy people living in the North. Methods. The research was conducted with respect for the main standards of biomedical ethics. For performance of a goal two groups of the examined persons were allocated: with a neutropenia (<2,0 x 109 Cells/l) and with the physiological level of neutrophils in a peripheral blood (2,5-5,0 x 109 Cells /l). In the blood smears painted according to Romanovsky-Gimza leukogram, neutrogram, monocytogram, lymphocytogram and phagocytic activity of neutrocytes were studied. Determination of cytokine concentrations of interferon-gamma (IFN-y) and granulocyte colony-stimulating factor (G-CSF) in serum was performed by enzyme-linked immunosorbent assay. Normality testing of quantitative indicators distribution was carried out using the Shapiro-Wilk test. The reliability of the differences between the groups was assessed using the Student's t-test and using the nonparametric Mann-Whitney method. Results. In the examined people, the frequency of neutropenia recording is 12,98 ± 0,27 %. It has been established that apoptosis of circulating neutrophils did not play a major role in the formation of neutropenia in the inhabitants of northern territories. With neutropenia, the content of segmented neutrophils is reduced, the frequency of detecting a shift to the left is higher (41,18 ± 3,75 versus 28,09 ± 0,84 %). In parallel with neutropenia, the monocyte and lymphocyte content decreases against the background of increased concentrations of IFN-y and G-CSF. Conclusions. Neutropenia in the inhabitants of northern territories is a consequence of increased activity of migration processes of blood cells. Reduction of the neutrophilic granulocyte content in the peripheral blood initiates the activation of neutrophil proliferation and their antibody-dependent cytotoxicity.

About the authors

S N Balashova

Federal Center for Integrated Arctic Research Russian Academy of Sciences

Email: balashovasn@mail.ru
кандидат биологических наук, старший научный сотрудник Института физиологии природных адаптаций 163000, г. Архангельск, пр. Ломоносова, д. 249

L K Dobrodeeva

Federal Center for Integrated Arctic Research Russian Academy of Sciences

163000, г. Архангельск, пр. Ломоносова, д. 249

References

  1. Балашова С. Н. Состояние апоптоза нейтрофилов периферической крови: автореф. дис.. канд. биол. наук. Архангельск, 2013. 18 с.
  2. Добродеева Л. К. Иммунологическое районирование. Сыктывкар: Коми НЦ УрО РАН, 2001. 112 с.
  3. Карякина О. Е., Добродеева Л. К. Взаимосвязь шеддинга рецепторов лимфоцитов с параметрами иммунологической реактивности у жителей Севера // Экология человека. 2016. № 11. С. 29-34.
  4. Кассирский И. А., Денщикова Д. И. Физиологические нормы лейкоцитов и проблема leucopenia innocens. М.: Медицина, 1974. 297 с.
  5. Кашутин С. Л., Пустынная М. В., Гудков А. Б., Данилов С. И., Ключарева С. В., Пирятинская В. А. Уровень экспрессии молекул адгезии на моноцитах в зависимости от морфологической дифференцировки их ядер // Клиническая лабораторная диагностика. 2014. Т. 59, № 10. С. 21-22.
  6. Нестерова И. В., Ковалева С. В., Чудилова Г. А., Ломтатидзе Л. В., Евглевский А. А. Двойственная роль нейтрофильных гранулоцитов в реализации противоопухолевой защиты // Иммунология. 2012. № 5. С. 281-287.
  7. Нехаев С. Г., Григорьев Ю. И. Полиморфноядерные лейкоциты как система антиэндотоксиновой защиты организма // Иммунология. 2010. № 3. С. 116-118.
  8. Смирнова А. Г., Колбай И. С., Образова А. Т. Содержание иммунных факторов в крови у беременных женщин в зависимости от условий проживания // Материалы 2-й международной конференции «Иммунофизиология: естественный аутоиммунитет в норме и патологии». Москва, 26-28 сентября, 2008. С. 193-194.
  9. Темл Х., Диам Х., Хаферлах Т. Атлас по гематологии / пер. с англ.; под общ. ред. проф. В. С. Камышникова. М.: МЕДпресс-информ, 2010. 208 с.
  10. Borregaard N., Cowland J. B. Granules of the human neutrophilic polymorphonuclear leukocytes // Blood. 1997. Vol. 89. Р 3503-3521.
  11. Braynsteiner H. Physiologie und Physiopathologie der weissen Blutzellen. Stuttgard, 1959. 346 p.
  12. Butcher S. K., Chahal H., Nayak L. Senescence in innate immune responses: reduced neutrophil phagocytic capacity and CD16 expression in elderly humans // J. Leukoc. Biol. 2001. Vol. 70. Р. 881-886.
  13. Chen Y., Wu H., Winnall W. et al. Tumor necrosis factor-alpha stimulates human neutrophyls to release preformed activin A // Immunol. and Cell Biol. 2011. Vol. 89, N 8. P. 889-896.
  14. Conssens L. M., Werb Z. Inflammatory cells and cancer: Think different // J. Exp. Med. 2001. Vol. 193. P. 23-26.
  15. Ford W. L., Gowans J. L. The Traffic of Lymphocytes // Seminars Hemat. 1969. Vol. 6. Р 67-83.
  16. Helden N. J., Williams J. M., Morgan M. D, Challa A. ANCA-stimulated neutrophils release BLyS and promote B cell survival // Ann. Rheum. Diseases. 2011. Vol. 70. P. 2229-2233.
  17. Huizinga T. W., Van der Schoot C. E., Roos D., Weening R. S. Induction of neutrophil Fcgamma receptor I expression can be used as a marker for biological activity of recombinant interferon-gamma in vivo // Blood. 1991. Vol. 77. P. 2088-2090.
  18. Megiovanni A. M., Francoise S., Macarena R. et al. Polymorphonuclear neutrophils deliver activation signals and antigenic molecules to dendritic cells: а new link between leukocytes upstream of T lymphocytes // J. Leukoc. Biol. 2006. Vol. 79. Р. 977-988.
  19. Meuret G., Hoffman G. Monocyte kinetic studies in normal and disease states // Brit. J. Hematol. 1973. Vol. 24. P. 275-279.
  20. Ottonello L., Epstein A. L., Mancini M. et al. Monoclonal LYM-1 antibody-dependent cytotocsis by human neutrophils exposed to GM-CSF: auto-regulation of target cell attack by catapsin G // J. Leukoc. Biol. 2004. Vol. 75. N 1. P 99-105.
  21. Weverka J. L., Dale D. C. Colony-stimulating factor in patients with chronic neutropenia // Blood. 1974. Vol. 43. Р 861-867

Copyright (c) 2018 Human Ecology


 


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies