THE ROLE OF ADHESION MOLECULES IN THE MIGRATION OF LEUKOCYTES FROM THE BLOOD TO THE CAPILLARIES OF PAPULES IN PATIENTS WITH PSORIASIS

The processes of leukocytes' migration to the psoriatic papule are important mechanisms in the development of immune reactive changes. The research relevance is determined by search for clinically significant targets for anti-inflammatory therapy (for ex. adhesion molecules). The aim of the study was to investigate the role of adhesion molecules in the mechanisms of blood leukocytes migration to papule capillaries in patients with psoriasis. Methods: Clinical immunological examination of 82 patients suffering from psoriasis vulgaris and exudative and 50 practically healthy people (a control group) was carried out. The content of neutrophils, monocytes, and lymphocytes was determined on the smears of venous blood and blood obtained as a phenomenon of pinpoint bleeding of the psoriatic triad localized on forearm. Their morphological differentiation was performed. The number of neutrophils, monocytes and lymphocytes of venous blood containing molecules of L-selectin (CD62L), LFA-1 (CD11a), LFA-3 (CD58), ICAM-1 (CD54), PECAM-1- (CD31) was determined on a flow cytometer FC-500, BeckmanCoulter (USA). Results: It was stated that the capillary blood of psoriatic papules was characterized by a lower content of neutrophils with a predominance of 2-segmental forms; the content of monocytes was 3 times higher than the level of venous blood due to an increase in the level of polymorphonuclear forms; as well as an increase in the number of large lymphocytes. At the stage of "rolling" an increasing tendency to the concentration of neutrophils carrying the l-selectin molecule were observed in patients with psoriasis. A decrease in the expression of ICAM-1 and LFA-3 molecules on neutrophils involved in the "stabile adhesion" stage was revealed. A statistically significant decrease in the concentration of PECAM-1 molecule on neutrophils was stated at the "transmigration" stage. Conclusions: The mechanisms of adhesion molecule expression in different leukocyte populations differ, which ensures accurate adjustment of migration through the endothelium. These reactions lead to the progression of immune inflammation, each stage of which has its own markers, such as cellular adhesion molecules.

neutrophils, lymphocytes, monocytes, adhesion molecules, psoriasis