Blood coagulation system in experimental acute lung injury and its treatment with dexamethasone

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The effects of short- and long-term administrations of dexamethasone on survival, severity of pulmonary edema, and hemostasis on experimental lipopolysaccharide-induced acute lung injury in rats were analyzed. Acute lung injury in rats was modeled by the intratracheal injection of lipopolysaccharide from the Salmonella enterica cell wall. White male rats were randomly divided into nine groups: the intact group consisted of 10 animals; two control groups of 20 animals each, in which acute lung injury was simulated without further treatment and removed from the experiment on day 3 or 7; six comparison groups of 20 animals each, in which, 3 h after modeling of acute lung injury and then once a day for 3 days (short mode of administration) or 7 days (long mode of administration), dexamethasone solution was administered intraperitoneally in the following doses: 0. 52 (equivalent to 6 mg/day for humans), 1. 71 (20 mg/day for humans), and 8 mg/kg/day (94 mg/day, pulse therapy for humans). On days 3 and 7, the survival rate, coagulogram values (active partial thromboplastin time, prothrombin time, activity of antithrombin, and soluble fibrin monomer complexes), and low-frequency piezotromboelastography data were assessed in the surviving animals. The results revealed that dexamethasone reduces mortality in acute lung injury and has a dose-dependent effect on the hemostasis system: with an increase in the dose administered, blood clotting processes increase and fibrinolysis is inhibited. Low-frequency piezothromboelastography with a conventional coagulogram allows for a comprehensive assessment of the hemostasis system, identifying violations, and timely drug correction.

作者简介

Nikita Voloshin

Kirov Military Medical Academy

编辑信件的主要联系方式.
Email: nikitavoloshin1990@gmail.com
ORCID iD: 0000-0002-3880-9548
SPIN 代码: 6061-4342
Scopus 作者 ID: 57926549500
Researcher ID: HSG-7925-2023

adjunct

俄罗斯联邦, Saint Petersburg

Evgeny Chuchalin

Kirov Military Medical Academy

Email: cheuspb@gmail.com
ORCID iD: 0000-0002-4162-910X
SPIN 代码: 7584-4729
Researcher ID: GZA-7499-2022

cadet

俄罗斯联邦, Saint Petersburg

Victoria Pugach

State Scientific Research Testing Institute of Military Medicine

Email: gniiivm_7@mil.ru
ORCID iD: 0000-0003-4290-350X
SPIN 代码: 3739-3699
Scopus 作者 ID: 57195201128
Researcher ID: ACD-0418-2022

MD, Cand. Sci. (Biol. ), senior research associate

俄罗斯联邦, Saint Petersburg

Vladimir Salukhov

Kirov Military Medical Academy

Email: vlasaluk@yandex.ru
ORCID iD: 0000-0003-1851-0941
SPIN 代码: 4531-6011
Scopus 作者 ID: 55804184100

MD, Dr. Sci. (Med.), assistant Professor

俄罗斯联邦, Saint Petersburg

Mikhail Tyunin

State Scientific Research Testing Institute of Military Medicine

Email: Tuynin84@yandex.ru
ORCID iD: 0000-0002-6974-5583
SPIN 代码: 6161-7029
Scopus 作者 ID: 25032311600

MD, Cand. Sci. (Med.)

俄罗斯联邦, Saint Petersburg

Mikhail Kharitonov

Kirov Military Medical Academy

Email: micjul11@yandex.ru
ORCID iD: 0000-0002-6521-7986
SPIN 代码: 7678-2278
Scopus 作者 ID: 57521284600
Researcher ID: H-6056-2015

MD, Dr. Sci. (Med.), Professor

俄罗斯联邦, Saint Petersburg

Yuri Rudakov

Kirov Military Medical Academy

Email: rudakov_yura@mail.ru
ORCID iD: 0000-0001-7914-6173
SPIN 代码: 5864-3853

MD, Cand. Sci. (Med.), assistant Professor

俄罗斯联邦, Saint Petersburg

Alexey Minakov

Kirov Military Medical Academy

Email: minakom@mai.ru
ORCID iD: 0000-0003-1525-3601
SPIN 代码: 5344-7883
Scopus 作者 ID: 57926549400
Researcher ID: HSG-9445-2023

adjunct

俄罗斯联邦, Saint Petersburg

Yuriy Goverdovskiy

Kirov Military Medical Academy

Email: goverdoc@yandex.ru
ORCID iD: 0000-0003-1241-9725
SPIN 代码: 2605-7097

MD, Dr. Sci. (Med.), Professor

俄罗斯联邦, Saint Petersburg

Tatyana Belyakova

Kirov Military Medical Academy

Email: tanyarus69@mail.ru
ORCID iD: 0009-0004-6005-733X
SPIN 代码: 8863-6596
Researcher ID: ISB-1403-2023

cadet

Saint Petersburg

Viktoriya Kochukova

Kirov Military Medical Academy

Email: kochukova.vika@mail.ru
SPIN 代码: 6263-3600
Researcher ID: ISB-2732-2023

cadet

俄罗斯联邦, Saint Petersburg

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