Decellularized matrix of the small intestine. Technology for obtaining, evaluating and using directions

A. A. Kokorina; Кокорина А. А.; S. V. Kromsky; Кромский С. В.; A. V. Kriventsov; Кривенцов А. В.; E. V. Mikhailova; Михайлова Е. В.; N. V. Pak; Пак Н. В.; A. A. Kondratenko; Кондратенко А. А.; L. P. Sigareva; Сигарева Л. П.; V. S. Sidorin; Сидорин В. С.; O. V. Kostina; Костина О. В.; L. S. Onishchenko; Онищенко Л. С.; V. N. Aleksandrov; Александров В. Н.

doi:10.17816/brmma62811

Decellularized matrix of the small intestine. Technology for obtaining, evaluating and using directions

Authors: Kokorina A.A.¹, Kromsky S.V.², Kriventsov A.V.¹, Mikhailova E.V.², Pak N.V.¹, Kondratenko A.A.¹, Sigareva L.P.², Sidorin V.S.¹, Kostina O.V.¹, Onishchenko L.S.¹, Aleksandrov V.N.¹^,2
Affiliations:
1. Military medical academy of S.M. Kirov
2. Saint-Petersburg State Pediatric Medical University
Issue: Vol 22, No 4 (2020)
Pages: 87-90
Section: Experimental trials
URL: https://journals.rcsi.science/1682-7392/article/view/62811
DOI: https://doi.org/10.17816/brmma62811
ID: 62811

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Abstract

Matrix (scaffold, matrix, framework, template) is a bioresorbable or non-bioresorbable material that can be filled with stem or somatic cells in/ex vivo in order to obtain a tissue-engineering structure for restoration of a lost organ, part of an organ, tissue. Scaffold must be to the extent necessary strong, non-immunogenic, bioactive. The porosity of the matrix must be open, the surface is rough and, most importantly, the matrix must contain factors of chemotaxis of endo- or exogenous origin, cell adhesion of their proliferation, differentiation. In this context, on the example of creating a decellularized small intestine matrix, a number of fundamental issues are highlighted regarding the choice of matrix material, its production technology, matrix evaluation in accordance with the criteria that correspond to the matrix for tissue engineering, and possible directions for its use. As a result, a non-immunogenic extracellular matrix of the small intestine was obtained by the method of detergent-enzymatic perfusion decellularization, which was sufficient in characteristics for use in various areas of tissue engineering, including plasty of defects of the skin, mucous membranes, small intestine, etc.

Keywords

tissue engineering, matrix, graft, scaffold, small intestine, short bowel syndrome, acellular matrix, decellularization, biomaterials

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About the authors

A. A. Kokorina

Military medical academy of S.M. Kirov

Author for correspondence.
Email: vmeda-nio@mil.ru
Russian Federation, Saint Petersburg