Development of a method for the identification of rs6265 polymorphism in the human brain neurotrophic factor gene
- Authors: Kutelev G.G.1, Krivoruchko A.B.1, Trandina A.E.1, Morozova N.E.2, Cherkashin D.V.1, Ivanov A.M.1, Ovchinnikov D.V.1, Glushakov R.I.1
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Affiliations:
- Military Medical Academy named after S.M. Kirov of the Ministry of Defense of the Russian Federation
- Peter the Great Saint Petersburg Polytechnic University
- Issue: Vol 23, No 4 (2021)
- Pages: 63-70
- Section: Clinical trials
- URL: https://journals.rcsi.science/1682-7392/article/view/80926
- DOI: https://doi.org/10.17816/brmma80926
- ID: 80926
Cite item
Abstract
The analysis and generalization of data from the literature sources characterizing the structural organization, regulation of expression, and functional activity of the neurotrophic factor of the human brain, which is one of the most common regulators of biological processes in the nervous system, is carried out. The results of numerous studies demonstrating the association of the brain-derived neurotrophic factor (BDNF) gene with the pathophysiology of the affective disorders are noted, and its contribution to the development of neuroplasticity is confirmed. The results of the design of a pair of primers and adaptation of the amplification reaction of the BDNF region with a length of 433 nucleotide pairs containing the polymorphic locus RS6265 are presented. Restriction endonuclease was selected. The sequence of primers, their localization, and correlation with the restriction site provided the separation of alternative alleles necessary for the successful identification of this marker. The use of the proposed technique made it possible to uniquely identify the genotype in 38 examined whole blood samples and identify the rare allelic variants. Also, the frequency of polymorphic variants of RS6265 of the BDNF gene was established in all the samples. There was an increase in the proportion of genotypes G/A and A/A of the RS6265 polymorphism of the BDNF gene in the group of examined systematically exposed to extreme factors. Identification of people with a rare A/A genotype of the RS6265 polymorphic locus of the BDNF gene is of a great importance for the monitoring system of long-term potentiation processes leading to the development of neuropsychic pathology. The possibility of implementing this method of genotyping in a typical laboratory using a polymerase chain reaction is proved. The proposed version of the polymerase chain reaction with the subsequent analysis of the polymorphism of the lengths of restriction fragments could be used as a fast, inexpensive, and reliable system for identifying single-nucleotide genetic polymorphisms.
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##article.viewOnOriginalSite##About the authors
Gennady G. Kutelev
Military Medical Academy named after S.M. Kirov of the Ministry of Defense of the Russian Federation
Author for correspondence.
Email: gena08@yandex.ru
ORCID iD: 0000-0002-6489-9938
SPIN-code: 5139-8511
candidate of medical sciences
Russian Federation, Saint PetersburgAlexander B. Krivoruchko
Military Medical Academy named after S.M. Kirov of the Ministry of Defense of the Russian Federation
Email: krab25@mail.ru
ORCID iD: 0000-0003-2035-4888
SPIN-code: 1324-0239
candidate of medical sciences
Russian Federation, Saint PetersburgAlexandra E. Trandina
Military Medical Academy named after S.M. Kirov of the Ministry of Defense of the Russian Federation
Email: sasha-trandina@rambler.ru
ORCID iD: 0000-0003-1875-1059
SPIN-code: 6089-3495
Russian Federation, Saint Petersburg
Natalia E. Morozova
Peter the Great Saint Petersburg Polytechnic University
Email: natusmorozovna@gmail.ru
ORCID iD: 0000-0002-3001-2593
SPIN-code: 4716-8586
Scopus Author ID: 56454006100
ResearcherId: A-7955-2014
candidate of biology sciences
Russian Federation, Saint PetersburgDmitriy V. Cherkashin
Military Medical Academy named after S.M. Kirov of the Ministry of Defense of the Russian Federation
Email: cherkashin_dmitr@mail.ru
ORCID iD: 0000-0003-1363-6860
SPIN-code: 2781-9507
doctor of medical sciences, professor
Russian Federation, Saint PetersburgAndrey M. Ivanov
Military Medical Academy named after S.M. Kirov of the Ministry of Defense of the Russian Federation
Email: iamvma@mail.ru
ORCID iD: 0000-0002-8899-7524
SPIN-code: 6971-1744
Scopus Author ID: 55867182100
doctor of medical sciences, professor
Russian Federation, Saint PetersburgDmitrii V. Ovchinnikov
Military Medical Academy named after S.M. Kirov of the Ministry of Defense of the Russian Federation
Email: dv.ovchinnikov-vma@yandex.ru
ORCID iD: 0000-0001-8408-5301
SPIN-code: 5437-3457
Scopus Author ID: 36185599800
candidate of medical sciences, associate professor
Russian Federation, Saint PetersburgRuslan I. Glushakov
Military Medical Academy named after S.M. Kirov of the Ministry of Defense of the Russian Federation
Email: glushakovruslan@gmail.ru
ORCID iD: 0000-0002-0161-5977
SPIN-code: 6860-8990
Scopus Author ID: 55263592100
candidate of medical sciences
Russian Federation, Saint PetersburgReferences
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