Features of microcirculation and metabolism in the skin and soft tissues of the injured area in experimental explosive trauma

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Abstract

Time, nature, and duration of changes in microcirculation and metabolism, as well as their differences in skin and muscle tissue of the injured area during experimental explosive trauma in rats in different wound process phases, are evaluated. Experimental explosive damage was simulated on 30 Wistar rats. The total condition of rats, their activity, interest in food and water, wound area with characteristic wound healing time calculation, the volume of injured pelvic limb, and changes of microcirculation and metabolism in the skin and skeletal muscles of the paravulnar region were evaluated. The explosive damage has led to a deterioration of microcirculation and metabolism in the skin, and especially, in the muscles of the injured area. Compared to the intact group, the microcirculation deterioration resulted in a decreased constant component of perfusion in the skin and muscles by 57.6% and 40.9% and a decreased vial by 76.9% and 76.5%, respectively (p < 0.05), as well as in reducing the fluorescent oxygen intake in the skin and muscles by 25.7% and 51.8% and a complex indicator of effective oxygen exchange by 81.1% and 91.9%, respectively (p < 0.05). During the experiment, the microcirculation and metabolism were gradually restored, which is more pronounced in the skin, except for the repeated deterioration of the non-vascular regulation of microcirculation in the muscle (a decreased vial by 29.3% of the norm, p < 0.05). Changes in the main indicators of microcirculation and metabolism indicate normal skin defect healing and unsatisfactory muscle defect repair (decreased volume of the injured limb (68% of the norm, p < 0.05)), accompanied by the recurrence of extravascular disorders in the muscle. Developing new and improved existing methods of delivering biologically active drugs and drugs to the area of muscular damage in the early days after the injury, which strengthen the local blood flow and create conditions for damaged muscle regeneration, reduce the wound healing time without forming pathological scars.

About the authors

Igor A. Shperling

The State Research Testing Institute of Military Medicine of the Ministry of Defense of the Russian Federation

Email: gniiivm_2@mil.ru
ORCID iD: 0000-0002-7029-8602
SPIN-code: 7730-4120

doctor of medical sciences, professor

Russian Federation, Saint Petersburg

Sergey O. Rostovtsev

The State Research Testing Institute of Military Medicine of the Ministry of Defense of the Russian Federation; The Military Academy of Logistics named after Army General A.V. Khrulev of the Ministry of Defense of the Russian Federation

Author for correspondence.
Email: chitah_serge@live.com
ORCID iD: 0000-0002-1037-5848
SPIN-code: 7978-5734

applicant

Russian Federation, Saint Petersburg; Saint Petersburg

Alexander V. Shulepov

The State Research Testing Institute of Military Medicine of the Ministry of Defense of the Russian Federation

Email: gniiivm_2@mil.ru
ORCID iD: 0000-0002-6134-809X
SPIN-code: 6197-0036

candidate of medical sciences

Russian Federation, Saint Petersburg

Anton S. Kourov

The State Research Testing Institute of Military Medicine of the Ministry of Defense of the Russian Federation; Saint Petersburg Research Institute of Ambulance named after I.I. Dzhanelidze

Email: gniiivm_2@mil.ru
ORCID iD: 0000-0001-6905-2501
SPIN-code: 4833-8746

applicant

Russian Federation, Saint Petersburg; Saint Petersburg

Michail V. Bazhenov

The State Research Testing Institute of Military Medicine of the Ministry of Defense of the Russian Federation

Email: gniiivm_2@mil.ru
ORCID iD: 0000-0003-2201-3948
SPIN-code: 5806-5250

head of the gospital

Russian Federation, Saint Petersburg

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Supplementary files

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2. Fig. 1. Simulation of experimental explosive damage: a — explosive wound (macropreparation); b — explosive wound with a millimeter ruler

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3. Fig. 2. The scheme of measuring the wound defect and the damaged pelvic limb volume of a rat: a–the wound defect size; b–the pelvic limb size (side view); c–the pelvic limb size (front view)

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4. Fig. 3. Methods of laser Doppler flowmetry and fluorescence diagnostics in rats: a — multifunctional complex of laser Doppler flowmetry “LAKK-M”; b — the size of the pelvic limb (side view); c — the sensor of the device installed on the skin/muscle of the paravular region

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5. Fig. 4. Phases of wound process in rats with experimental explosive damage to soft tissues (the numbers in the columns indicate the average duration in days)

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Copyright (c) 2022 Shperling I.A., Rostovtsev S.O., Shulepov A.V., Kourov A.S., Bazhenov M.V.

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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