Optimization of diagnosis of SARS-CoV-2 infection using polymerase chain reaction in a large multi-specialty hospital

K. V. Zhdanov; Жданов К. В.; K. V. Kozlov; Козлов К. В.; Yu. I. Bulankov; Буланьков Ю. И.; S. M. Zakharenko; Захаренко С. М.; I. M. Uliukin; Улюкин И. М.; G. G Zagorodnikov; Загородников Г. Г; S. G. Grigoriev; Григорьев С. Г.; E. S. Orlova; Орлова Е. С.; A. A Shuklina; Шуклина А. А; A. M. Ivanov; Иванов А. М.; O. V. Maltsev; Мальцев О. В.; V. S. Sukachev; Сукачев В. С.; K. V. Kasyanenko; Касьяненко К. В.; N. E. Grinchenko; Гринченко Н. Е.

doi:10.17816/brmma50036

Optimization of diagnosis of SARS-CoV-2 infection using polymerase chain reaction in a large multi-specialty hospital

Authors: Zhdanov K.V.¹, Kozlov K.V.¹, Bulankov Y.I.¹, Zakharenko S.M.¹, Uliukin I.M.¹, Zagorodnikov G.G¹, Grigoriev S.G.¹, Orlova E.S.¹, Shuklina A.A¹, Ivanov A.M.¹, Maltsev O.V.¹, Sukachev V.S.¹, Kasyanenko K.V.¹, Grinchenko N.E.¹
Affiliations:
1. Military Medical Academy. S. M. Kirov
Issue: Vol 22, No 2 (2020)
Pages: 7-10
Section: Articles
URL: https://journals.rcsi.science/1682-7392/article/view/50036
DOI: https://doi.org/10.17816/brmma50036
ID: 50036

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Abstract

Аbstract. We consider the possibility of optimizing the diagnosis of infection caused by SARS-CoV-2 using polymerase chain reaction in a multi-specialty hospital, repurposed for the treatment of COVID-19 patients, using the example Of the military medical Academy named after S.M. Kirov. The analysis of scientific publications selected in accordance with the purpose of the study, analyzed data from 218 samples of polymerase chain reaction in patients with COVID-19, who were in the clinics Of the military medical Academy named after Sm. Time intervals were established depending on the clinical forms and severity of the infectious process, in which the probability of a positive and negative result of a polymerase chain reaction to SARS-CoV-2 RNA was maximum and minimum. It was shown that during the examination and treatment, molecular biological diagnostics were performed excessively (4 times in more than 50% of patients) and in 97,3% of patients unreasonably early (8,5±0,4 days). At the same time, the highest frequency of negative results of polymerase chain reaction to SARS-CoV-2 RNA was observed in the period from 9 to 10 and from 12 to 14 days, while it depended on the clinical form and severity of the infectious process. In this regard, the volume diagnosis using polymerase chain reaction should be reduced and to conduct research when entering the hospital, on the 9th-10th day (in patients inapparently forms and acute respiratory diseases, lung flow) and 12-14 days before discharge in patients with moderate and severe course of the infectious process.

Keywords

coronavirus, acute respiratory diseases, virus genetic material, molecular and biological diagnostics, multiplex polymerase chain reaction in real time, multidisciplinary hospital of the Kirov Military medical Academy