Email this article IMMUNOLOGICAL MECHANISMS OF ANTI-INFLAMMATORY ACTION OF MODIFIED YELLOW CLAY IN THE EXPERIMENT
- Authors: Sagradyan Gayane Valerievna G.1,2, Abramtsova A.V.3
-
Affiliations:
- 1Federal State Budgetary Institution "North Caucasian Federal Scientific and Clinical Center of Federal Medical and Biological Agency"
- 2Рyatigorsk Medical Pharmaceutical Institute – branch of Volgograd State Medical University
- Federal State Budgetary Institution "North Caucasian Federal Scientific and Clinical Center of the Federal Medical and Biological Agency"
- Issue: Vol 21, No 2 (2022)
- Pages: 107-113
- Section: Original studies
- URL: https://journals.rcsi.science/1681-3456/article/view/109297
- ID: 109297
Cite item
Open Access
Access granted
Subscription Access
Abstract
BACKGROUND. One of the tasks of experimental balneology is the study of little-studied natural sources and the production of modified balneological remedies used in diseases of the musculoskeletal system.
AIMS: is to evaluate immunological mechanisms of anti-inflammatory action of external balneological medicine based on natural clays to study their therapeutic effectiveness in diseases of musculoskeletal system in the adjuvant arthritis model.
MATERIAL AND METHODS: An experimental study was conducted with the participation of 79 laboratory animals (male rats, Wistar line) distributed by simple sampling into 5 groups: in the control group 1 (n = 15) there were healthy animals (intact); in the 2nd group (n = 13) the animals had adjuvant arthritis (AA) without exposure; the 3rd group (n = 17) had an AA model and Tambukan dirt; in the 4th group (n = 17) there was model AA and a course of yellow clay, the 5th group (n = 17) had model AA and a course of modified clay. The anti-inflammatory effects of native and modified external balneological medicine were examined by evaluating the response of immune-competent cells of peripheral blood in reproducing chronic inflammation in AA model.
RESULTS: The conducted studies have shown that the external use of modified yellow clay in the form of balneological medicine “Glinofir” is comparable to the effect of Tambukan dirt. According to the immune marker analysis there was a significant change in population lymphocyte structure in peripheral blood as a result of AA modeling, namely, the number of B lymphocytes increased (2.5 times; p < 0.02) with simultaneous reduction of total T-lymphocytes (2.5 times; p < 0.05); the level of circulating immune complexes showed a tendency to increase with the animals of the group with AA model without treatment (59.8 ± 4.18 units in AA and 52.4 ± 3.44 units with healthy animals; p = 0.1).
CONCLUSION: According to the results of the experimental studies, “Glinofir” which has immune-modulatory and anti-inflammatory effects can be recommended for subsequent introduction into clinical practice as the treatment and medical rehabilitation of patients with diseases of musculoskeletal system.
Full Text
##article.viewOnOriginalSite##About the authors
Gayane Sagradyan Gayane Valerievna
1Federal State Budgetary Institution "North Caucasian Federal Scientific and Clinical Center of Federal Medical and Biological Agency"; 2Рyatigorsk Medical Pharmaceutical Institute – branch of Volgograd State Medical University
Email: zamoms@skfmba.ru
ORCID iD: 0000-0001-7970-1958
SPIN-code: 9629-5773
Scopus Author ID: 57204624456
Кандидат медицинских наук,старший научный сотрудник ОИМДФФ ПНИИК ФФГБУ СКФНКЦ ФМБА России в г. Пятигорске
Russian Federation, 357500, Pyatigorsk, Kirov Avenue, 30 357500, Pyatigorsk, Kalinin Avenue,11Anna Viktorovna Abramtsova
Federal State Budgetary Institution "North Caucasian Federal Scientific and Clinical Center of the Federal Medical and Biological Agency"
Author for correspondence.
Email: a.v.abramtsova@skfmba.ru
ORCID iD: 0000-0001-7435-6286
SPIN-code: 9608-8004
Scopus Author ID: 57194732233
Candidate of Medical Sciences, Senior Researcher Russian Federation, 357500, Pyatigorsk, Kirov Avenue, 30
References
- Kulikov V.Yu. Sanogenetic mechanisms of pelotherapy. Novosibirsk: From NSMU. 1999. (in Russ.)
- Leshchinsky A.F., Zuza Z.I. Peloid and pharmacotherapy in inflammatory diseases. Kyiv: Health”I. 1985.
- Popov S.V., Mihajlov V.P. Acute period of adjuvant arthritis: features of the development of inflammation and changes in the rheological properties of blood. Regionarnoe krovoobrashchenie i mikrocirkulyaciya [Regional circulation and microcirculation]. 2007; 5: 53-57. (in Russ.)
- Sizova L.V. Influence of various methods of treatment on indicators of quality of life in patients with osteoarthritis [Dissertation] Orenburg. 2004. (in Russ.)
- Kurbanov V.A., Kajsinova A.S., Demeshko N.I., Abramcova A.V., Reps V.F. Influence of a preformed preparation from yellow clay in comparison with the therapeutic mud of the Tambukan lake on the course of adjuvant arthritis. Kurortnaya medicina [Resort medicine]. 2014; 4: 31-36. (in Russ.)
- Nejmark E.B., Eroshchev-SHak V.A., CHizhikova N.P., Kompanceva E.I. Interaction of Clay Minerals with Microorganisms: Review of Experimental Data. ZHurnal obshchej biologii [Journal of General Biology]. 2009; 70(2): 155–167. (in Russ.)
- Selected lectures on clinical rheumatology. Ed. by V.A. Nasonova, N.V. Bunchuk. M.: Medicine. 2001. (in Russ.)
- Potanin A.Yu. Early rheumatoid arthritis: clinical and immunological characteristics in various variants of the disease [Dissertation]. M. 2006. (in Russ.)
- Zhang X., Mao Z., Yu S. Suppression of early experimental osteoarthritis by gene transfer of interleukin-1 receptor antagonist and interleukin-10. J. Orthop. Res. 2004; 22 (4): 742-792.
- Abramcova A.V., Demeshko N.I., Reps V.F., Pigunova L.A., Kurbanov V.A. The effect of modified clay and natural therapeutic mud on the state of the blood oxygen transport system in experimental arthritis. Kurortnaya medicina [Resort medicine]. 2013; 2: 66-71. (in Russ.)
- Kurbanov V.A., Mal'chukovskij L.B., Danilov S.R., SHCHelkunov A.V., Vysochina E.I. Physical and chemical composition of steel-gray clay from the Novo-Pyatigorsk quarry of the foothill region of the Stavropol Territory and the preformed balneological agent "Glinofir" obtained from it. Kurortnaya medicina [Resort medicine]. 2012; 2: 19-26. (in Russ.)
- Balneological agent "Glinofir" for the treatment and prevention of various diseases: Pat. 2540396 Ros. Federation: A61K35/00. L.B. Malchukovsky and others; applicant and patentee Medical-ecological association "BIOTECH". No. 2013112129/15; dec. 03/19/2013; publ. 02/10/2015. (in Russ.)
- Sanatorium-and-spa treatment: national guidelines. Ed. A. N. Razumova, V. I. Starodubova, G. N. Ponomarenko. Moscow: GEOTAR-Media. 2021.Fizicheskaya i reabilitacionnaya medicina: nacional'noe rukovodstvo. Pod red. G.N. Ponomarenko. M.: GEOTAR-Media. 2020. (in Russ.)
- Khaidukov S.V., Zurochka A.V., Chereshnev V.A. Cytometric analysis in clinical immunology. Yekaterinburg, 2011. (in Russ.)
- Gashchkova V., Matl I., Kashlik I., Kogandrle V. Circulating Ag-At complexes in patients with immunocomplex diseases. CHekhoslovackaya medicina [Czechoslovak medicine]. 1978; 2: 117-120. (in Russ.)
Supplementary files
