Genomic mapping of chromatin proteins by using Daminv modification of an FLP-dependent DamID approach


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To identify interactions of chromatin proteins with the genome of the cell type of interest that is a part of heterologous tissues and organs of Drosophila, an FLP-dependent DamID approach was recently developed [4], which does not require sorting of cells or nuclei. Here, a modification of this approach, Daminv, is described. The modified approach was validated by generating the binding pattern of the LAM protein, a component of the inner membrane of the nuclear envelope, with the genome of glial cells of the Drosophila larval central brains.

Sobre autores

A. Pindyurin

Netherlands Cancer Institute; Institute of Molecular and Cellular Biology

Autor responsável pela correspondência
Email: a.pindyurin@mcb.nsc.ru
Países Baixos, Amsterdam, 1066 CX; Novosibirsk, 630090

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