Email this article Genomic mapping of chromatin proteins by using Daminv modification of an FLP-dependent DamID approach
- Authors: Pindyurin A.V.1,2
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Affiliations:
- Netherlands Cancer Institute
- Institute of Molecular and Cellular Biology
- Issue: Vol 472, No 1 (2017)
- Pages: 15-18
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/211801
- DOI: https://doi.org/10.1134/S1607672917010057
- ID: 211801
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Abstract
To identify interactions of chromatin proteins with the genome of the cell type of interest that is a part of heterologous tissues and organs of Drosophila, an FLP-dependent DamID approach was recently developed [4], which does not require sorting of cells or nuclei. Here, a modification of this approach, Daminv, is described. The modified approach was validated by generating the binding pattern of the LAM protein, a component of the inner membrane of the nuclear envelope, with the genome of glial cells of the Drosophila larval central brains.
About the authors
A. V. Pindyurin
Netherlands Cancer Institute; Institute of Molecular and Cellular Biology
Author for correspondence.
Email: a.pindyurin@mcb.nsc.ru
Netherlands, Amsterdam, 1066 CX; Novosibirsk, 630090
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