Enviar artigo por via de e-mail Mathematics analysis of polymerase chain reaction kinetic curves
- Autores: Sochivko D.G.1, Fedorov A.A.2, Varlamov D.A.3, Kurochkin V.E.2, Petrov R.V.4
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Afiliações:
- JSC Syntol
- Institute for Analytical Instrumentation
- All-Russia Research Institute of Agricultural Biotechnology
- National Research Center, Institute of Immunology
- Edição: Volume 466, Nº 1 (2016)
- Páginas: 13-16
- Seção: Biochemistry, Biophysics and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/210975
- DOI: https://doi.org/10.1134/S160767291601004X
- ID: 210975
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Resumo
The paper reviews different approaches to the mathematical analysis of polymerase chain reaction (PCR) kinetic curves. The basic principles of PCR mathematical analysis are presented. Approximation of PCR kinetic curves and PCR efficiency curves by various functions is described. Several PCR models based on chemical kinetics equations are suggested. Decision criteria for an optimal function to describe PCR efficiency are proposed.
Sobre autores
D. Sochivko
JSC Syntol
Email: faa@bk.ru
Rússia, ul. Timiryazevskaya 42, Moscow, 127550
A. Fedorov
Institute for Analytical Instrumentation
Autor responsável pela correspondência
Email: faa@bk.ru
Rússia, Rizhskii pr. 26, St. Petersburg, 190103
D. Varlamov
All-Russia Research Institute of Agricultural Biotechnology
Email: faa@bk.ru
Rússia, ul. Timiryazevskaya 42, Moscow, 127550
V. Kurochkin
Institute for Analytical Instrumentation
Email: faa@bk.ru
Rússia, Rizhskii pr. 26, St. Petersburg, 190103
R. Petrov
National Research Center, Institute of Immunology
Email: faa@bk.ru
Rússia, Kashirskoe sh. 24, Moscow, 115478
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