Мақаланы E-mail арқылы жіберу The Application of Recombinant Phototoxins 4D5scFv-miniSOG and DARPin-miniSOG to Study the HER2 Receptor Internalization
- Авторлар: Kuzichkina E.O.1,2, Shilova O.N.1, Deyev S.M.1, Petrov R.V.1
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Мекемелер:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Moscow Institute of Physics and Technology (State University)
- Шығарылым: Том 482, № 1 (2018)
- Беттер: 245-248
- Бөлім: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/212435
- DOI: https://doi.org/10.1134/S1607672918050046
- ID: 212435
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
It was found that the fluorescent properties of the phototoxic domain of miniSOG allow to assess the ability of toxins to bind to human breast adenocarcinoma cells SK-BR-3 and study the dynamics of their internalization. We established that the main cause of the decrease of the fluorescence intensity of the recombinant proteins 4D5scFv-miniSOG and DARPin-miniSOG during their internalization in the complex with the HER2 receptor is their shielding and absorption of the fluorescence of miniSOG by the cells fluorophores.
Авторлар туралы
E. Kuzichkina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry; Moscow Institute of Physics and Technology (State University)
Хат алмасуға жауапты Автор.
Email: kuzichkinazhenya@mail.ru
Ресей, Moscow, 117997; Dolgoprudnyi, Moscow oblast, 141700
O. Shilova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: kuzichkinazhenya@mail.ru
Ресей, Moscow, 117997
S. Deyev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: kuzichkinazhenya@mail.ru
Ресей, Moscow, 117997
R. Petrov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: kuzichkinazhenya@mail.ru
Ресей, Moscow, 117997
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