Email this article New Targets of Kunitz-Type Peptide from Sea Anemone Heteractis magnifica
- Authors: Kvetkina A.N.1, Kaluzhskiy L.A.2, Leychenko E.V.1,3, Isaeva M.P.1, Ivanov A.S.2, Kozlovskaya E.P.1
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Affiliations:
- Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch, Russian Academy of Sciences
- Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences
- Far East Federal University
- Issue: Vol 487, No 1 (2019)
- Pages: 260-263
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/213060
- DOI: https://doi.org/10.1134/S1607672919040033
- ID: 213060
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Abstract
The interaction of Kunitz-type peptide, HMIQ3c1, from the sea anemone Heteractis magnifica with several serine proteases, including inflammatory proteases, was investigated using the surface plasmon resonance approach. We showed that the recombinant analog of HMIQ3c1 forms sufficiently strong complexes with trypsin (KD = 1.07 × 10–9 М) and chymotrypsin (KD = 4.70 × 10–8 М). Analysis of thermodynamic parameters of HMIQ3c1/chymotrypsin revealed significant contribution of the entropic factor to the complex formation. The formation of specific complexes of HMIQ3c1 with the kallikrein (KD = 2.81 × 10–8 М) and neutrophil elastase (KD = 1.11 × 10–7 М) indicates its anti-inflammatory activity and makes prospects to use the peptide as a potential therapeutic agent.
About the authors
A. N. Kvetkina
Elyakov Pacific Institute of Bioorganic Chemistry,Far East Branch, Russian Academy of Sciences
Author for correspondence.
Email: kvetkinaan@gmail.com
Russian Federation, Vladivostok, 690022
L. A. Kaluzhskiy
Orekhovich Institute of Biomedical Chemistry,Russian Academy of Medical Sciences
Email: kvetkinaan@gmail.com
Russian Federation,
Pogodinskaya ul. 10, Moscow, 119832
E. V. Leychenko
Elyakov Pacific Institute of Bioorganic Chemistry,Far East Branch, Russian Academy of Sciences; Far East Federal University
Email: kvetkinaan@gmail.com
Russian Federation, Vladivostok, 690022; Vladivostok, 690022
M. P. Isaeva
Elyakov Pacific Institute of Bioorganic Chemistry,Far East Branch, Russian Academy of Sciences
Email: kvetkinaan@gmail.com
Russian Federation, Vladivostok, 690022
A. S. Ivanov
Orekhovich Institute of Biomedical Chemistry,Russian Academy of Medical Sciences
Email: kvetkinaan@gmail.com
Russian Federation,
Pogodinskaya ul. 10, Moscow, 119832
E. P. Kozlovskaya
Elyakov Pacific Institute of Bioorganic Chemistry,Far East Branch, Russian Academy of Sciences
Email: kvetkinaan@gmail.com
Russian Federation, Vladivostok, 690022
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