NAD(P)H:FMN‑Oxidoreductase Functioning Under Macromolecular Crowding: In Vitro Modeling
- Autores: Govorun A.E.1, Esimbekova E.N.2,1, Kratasyuk V.A.1,2
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Afiliações:
- Siberian Federal University
- Institute of Biophysics, Siberian Branch, Russian Academy of Sciences
- Edição: Volume 486, Nº 1 (2019)
- Páginas: 213-215
- Seção: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/213028
- DOI: https://doi.org/10.1134/S160767291903013X
- ID: 213028
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Resumo
The functioning of NAD(P)H:FMN‑oxidoreductase (Red) from Vibrio fischeri under conditions of macromolecular crowding (MMC) simulated in vitro by adding biopolymers (starch and gelatin) was studied. The dissociation rate constants and the activation energies of dissociation of Red to the subunits were calculated, and the process of denaturation of Red was analyzed. It is shown that the functioning of Red both under conditions of MMC and in diluted solutions is the same. This result refutes the common belief that the native conformation of enzymes in vivo is stabilized due to MMC as compared to the in vitro conditions.
Sobre autores
A. Govorun
Siberian Federal University
Autor responsável pela correspondência
Email: aebezrukih@gmail.com
Rússia, Krasnoyarsk, 660041
E. Esimbekova
Institute of Biophysics, Siberian Branch, Russian Academyof Sciences; Siberian Federal University
Email: aebezrukih@gmail.com
Rússia, Krasnoyarsk, 660036; Krasnoyarsk, 660041
V. Kratasyuk
Siberian Federal University; Institute of Biophysics, Siberian Branch, Russian Academyof Sciences
Email: aebezrukih@gmail.com
Rússia, Krasnoyarsk, 660041; Krasnoyarsk, 660036
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