NAD(P)H:FMN‑Oxidoreductase Functioning Under Macromolecular Crowding: In Vitro Modeling


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Resumo

The functioning of NAD(P)H:FMN‑oxidoreductase (Red) from Vibrio fischeri under conditions of macromolecular crowding (MMC) simulated in vitro by adding biopolymers (starch and gelatin) was studied. The dissociation rate constants and the activation energies of dissociation of Red to the subunits were calculated, and the process of denaturation of Red was analyzed. It is shown that the functioning of Red both under conditions of MMC and in diluted solutions is the same. This result refutes the common belief that the native conformation of enzymes in vivo is stabilized due to MMC as compared to the in vitro conditions.

Sobre autores

A. Govorun

Siberian Federal University

Autor responsável pela correspondência
Email: aebezrukih@gmail.com
Rússia, Krasnoyarsk, 660041

E. Esimbekova

Institute of Biophysics, Siberian Branch, Russian Academy
of Sciences; Siberian Federal University

Email: aebezrukih@gmail.com
Rússia, Krasnoyarsk, 660036; Krasnoyarsk, 660041

V. Kratasyuk

Siberian Federal University; Institute of Biophysics, Siberian Branch, Russian Academy
of Sciences

Email: aebezrukih@gmail.com
Rússia, Krasnoyarsk, 660041; Krasnoyarsk, 660036

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