The fluorescence lifetime of lipofuscin granule fluorophores contained in the retinal pigment epithelium cells from human cadaver eyes in normal state and in the case of visualized pathology
- Autores: Yakovleva M.A.1, Feldman T.B.1,2, Arbukhanova P.M.3, Borzenok S.A.3, Kuzmin V.A.1, Ostrovsky M.A.1,2
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Afiliações:
- Emanuel Institute of Biochemical Physics
- Biological Faculty
- Fedorov Eye Microsurgery Research and Technology Complex
- Edição: Volume 474, Nº 1 (2017)
- Páginas: 239-243
- Seção: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/211930
- DOI: https://doi.org/10.1134/S1607672917030231
- ID: 211930
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Resumo
A comparative analysis of fluorescence lifetime of lipofuscin granule fluorophores contained in the retinal pigment epithelium cells from human cadaver eyes in normal state and in the case of visualized pathology was carried out. Measurements of fluorescence lifetimes of bis-retinoids and their photooxidation and photodegradation products were carried out using the method of counting time-correlated photons. Comparative analysis showed that, in the case of visualized pathology, the contribution of photooxidation and photodegradation products of bis-retinoids to the total fluorescence of the retinal pigment epithelium cell suspension increases in comparison with the norm.
Sobre autores
M. Yakovleva
Emanuel Institute of Biochemical Physics
Autor responsável pela correspondência
Email: lina.invers@gmail.com
Rússia, Moscow, 119334
T. Feldman
Emanuel Institute of Biochemical Physics; Biological Faculty
Email: lina.invers@gmail.com
Rússia, Moscow, 119334; Moscow, 119991
P. Arbukhanova
Fedorov Eye Microsurgery Research and Technology Complex
Email: lina.invers@gmail.com
Rússia, Moscow, 127486
S. Borzenok
Fedorov Eye Microsurgery Research and Technology Complex
Email: lina.invers@gmail.com
Rússia, Moscow, 127486
V. Kuzmin
Emanuel Institute of Biochemical Physics
Email: lina.invers@gmail.com
Rússia, Moscow, 119334
M. Ostrovsky
Emanuel Institute of Biochemical Physics; Biological Faculty
Email: lina.invers@gmail.com
Rússia, Moscow, 119334; Moscow, 119991
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