Studying a novel ecdysone-dependent enhancer
- Autores: Nikolenko J.V.1, Krasnov A.N.1, Mazina M.Y.1, Georgieva S.G.1,2, Vorobyeva N.E.1
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Afiliações:
- Institute of Gene Biology
- Engelhardt Institute of Molecular Biology
- Edição: Volume 474, Nº 1 (2017)
- Páginas: 236-238
- Seção: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/211925
- DOI: https://doi.org/10.1134/S160767291703022X
- ID: 211925
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Resumo
Earlier [4], we developed a two-stage scheme of the activation of expression of the ecdysone cascade gene ftz-f1 in Drosophila melanogaster S2 cells. In the first intron of the ftz-f1 gene, we found a binding site for the transcription activator DHR3. In this work, we studied the properties of this genomic element: the change in the histone modification level at different stages of transcription activation were analyzed, and data indicating the interaction of the intron element with the promoter were obtained. Taken together, the results of this study indicate that the studied genomic element exhibits the properties of an enhancer and functions at the stage of active gene transcription.
Sobre autores
J. Nikolenko
Institute of Gene Biology
Autor responsável pela correspondência
Email: julia.v.nikolenko@gmail.com
Rússia, Moscow, 119334
A. Krasnov
Institute of Gene Biology
Email: julia.v.nikolenko@gmail.com
Rússia, Moscow, 119334
M. Mazina
Institute of Gene Biology
Email: julia.v.nikolenko@gmail.com
Rússia, Moscow, 119334
S. Georgieva
Institute of Gene Biology; Engelhardt Institute of Molecular Biology
Email: julia.v.nikolenko@gmail.com
Rússia, Moscow, 119334; Moscow, 119991
N. Vorobyeva
Institute of Gene Biology
Email: julia.v.nikolenko@gmail.com
Rússia, Moscow, 119334
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