Development and investigation of recombinant immunotoxin protein 4D5scFv-mCherry-PE(40)
- 作者: Shilova O.N.1, Souslova E.A.1, Pilunov A.M.1, Deyev S.M.1,2, Petrov R.V.1
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隶属关系:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Nizhny Novgorod State University
- 期: 卷 471, 编号 1 (2016)
- 页面: 450-453
- 栏目: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/211794
- DOI: https://doi.org/10.1134/S160767291606020X
- ID: 211794
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详细
Development of agents for theranostics implies combining the targeting module, the effector module, and the detection module within the same complex or recombinant protein. We have constructed, isolated, and characterized the 4D5scFv-mCherry-PE(40) protein, which exhibits fluorescent properties and specifically binds to cancer cells expressing the HER2 receptor and reduces their viability. The ability of the obtained targeted antitumor agent 4D5scFv-mCherry-PE(40) to selectively stain the HER2-positive cells and its highly selective cytotoxicity against these cells make the obtained targeted recombinant protein 4D5scFv-mCherry-PE(40) a promising theranostic agent for the diagnostics and therapy of HER2-positive human tumors.
作者简介
O. Shilova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
编辑信件的主要联系方式.
Email: olga.shilova.n@gmail.com
俄罗斯联邦, Moscow, 117997
E. Souslova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: olga.shilova.n@gmail.com
俄罗斯联邦, Moscow, 117997
A. Pilunov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: olga.shilova.n@gmail.com
俄罗斯联邦, Moscow, 117997
S. Deyev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry; Nizhny Novgorod State University
Email: olga.shilova.n@gmail.com
俄罗斯联邦, Moscow, 117997; Nizhny Novgorod, 603600
R. Petrov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: olga.shilova.n@gmail.com
俄罗斯联邦, Moscow, 117997
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