EPR spectroscopy solutions for assessment of decellularization of intrathoracic organs and tissues
- 作者: Gubareva E.A.1, Kuevda E.V.1, Dzhimak S.S.2, Basov A.A.1, Sotnichenko A.S.1, Bolotin S.N.2, Gilevich I.V.1, Gumenyuk I.S.1, Macchiarini P.1
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隶属关系:
- Kuban State Medical University
- Kuban State University
- 期: 卷 467, 编号 1 (2016)
- 页面: 113-116
- 栏目: Biochemistry, Biophysics and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/211045
- DOI: https://doi.org/10.1134/S1607672916020101
- ID: 211045
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详细
Using EPR spectroscopy it was established that the determination of the concentration of paramagnetic centers in lyophilized tissues allows indirect evaluation of the quality of decellularization of intrathoracic organs (diaphragm, heart, and lungs), since the content of paramagnetic particles in them can serve as a criterion of cell viability and points to the necessity to repeat decellularization. Experiments in rats showed that the EPR spectra of the native thoracic organs contained paramagnetic centers with g-factor values ranging from 2.007 to 2.011 at a concentration of 10–8 to 6.62 × 10–7 mol/g of lyophilized tissue, whereas in all decellularized tissues of the same organs paramagnetic particles were not detected.
作者简介
E. Gubareva
Kuban State Medical University
编辑信件的主要联系方式.
Email: g_lena82@list.ru
俄罗斯联邦, ul. Sedina 4, Krasnodar, 350000
E. Kuevda
Kuban State Medical University
Email: g_lena82@list.ru
俄罗斯联邦, ul. Sedina 4, Krasnodar, 350000
S. Dzhimak
Kuban State University
Email: g_lena82@list.ru
俄罗斯联邦, ul. Stavropol’skaya 149, Krasnodar, 350040
A. Basov
Kuban State Medical University
Email: g_lena82@list.ru
俄罗斯联邦, ul. Sedina 4, Krasnodar, 350000
A. Sotnichenko
Kuban State Medical University
Email: g_lena82@list.ru
俄罗斯联邦, ul. Sedina 4, Krasnodar, 350000
S. Bolotin
Kuban State University
Email: g_lena82@list.ru
俄罗斯联邦, ul. Stavropol’skaya 149, Krasnodar, 350040
I. Gilevich
Kuban State Medical University
Email: g_lena82@list.ru
俄罗斯联邦, ul. Sedina 4, Krasnodar, 350000
I. Gumenyuk
Kuban State Medical University
Email: g_lena82@list.ru
俄罗斯联邦, ul. Sedina 4, Krasnodar, 350000
P. Macchiarini
Kuban State Medical University
Email: g_lena82@list.ru
俄罗斯联邦, ul. Sedina 4, Krasnodar, 350000
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