EPR spectroscopy solutions for assessment of decellularization of intrathoracic organs and tissues
- Авторы: Gubareva E.A.1, Kuevda E.V.1, Dzhimak S.S.2, Basov A.A.1, Sotnichenko A.S.1, Bolotin S.N.2, Gilevich I.V.1, Gumenyuk I.S.1, Macchiarini P.1
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Учреждения:
- Kuban State Medical University
- Kuban State University
- Выпуск: Том 467, № 1 (2016)
- Страницы: 113-116
- Раздел: Biochemistry, Biophysics and Molecular Biology
- URL: https://journals.rcsi.science/1607-6729/article/view/211045
- DOI: https://doi.org/10.1134/S1607672916020101
- ID: 211045
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Аннотация
Using EPR spectroscopy it was established that the determination of the concentration of paramagnetic centers in lyophilized tissues allows indirect evaluation of the quality of decellularization of intrathoracic organs (diaphragm, heart, and lungs), since the content of paramagnetic particles in them can serve as a criterion of cell viability and points to the necessity to repeat decellularization. Experiments in rats showed that the EPR spectra of the native thoracic organs contained paramagnetic centers with g-factor values ranging from 2.007 to 2.011 at a concentration of 10–8 to 6.62 × 10–7 mol/g of lyophilized tissue, whereas in all decellularized tissues of the same organs paramagnetic particles were not detected.
Об авторах
E. Gubareva
Kuban State Medical University
Автор, ответственный за переписку.
Email: g_lena82@list.ru
Россия, ul. Sedina 4, Krasnodar, 350000
E. Kuevda
Kuban State Medical University
Email: g_lena82@list.ru
Россия, ul. Sedina 4, Krasnodar, 350000
S. Dzhimak
Kuban State University
Email: g_lena82@list.ru
Россия, ul. Stavropol’skaya 149, Krasnodar, 350040
A. Basov
Kuban State Medical University
Email: g_lena82@list.ru
Россия, ul. Sedina 4, Krasnodar, 350000
A. Sotnichenko
Kuban State Medical University
Email: g_lena82@list.ru
Россия, ul. Sedina 4, Krasnodar, 350000
S. Bolotin
Kuban State University
Email: g_lena82@list.ru
Россия, ul. Stavropol’skaya 149, Krasnodar, 350040
I. Gilevich
Kuban State Medical University
Email: g_lena82@list.ru
Россия, ul. Sedina 4, Krasnodar, 350000
I. Gumenyuk
Kuban State Medical University
Email: g_lena82@list.ru
Россия, ul. Sedina 4, Krasnodar, 350000
P. Macchiarini
Kuban State Medical University
Email: g_lena82@list.ru
Россия, ul. Sedina 4, Krasnodar, 350000
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