Immunomodulating activity of individual substanсes isolated from the aerial part of Rhaponticum uniflorum (L.) DC in experimental immunodeficiency
- Authors: Khobrakova V.B.1,2, Tugarina Y.A.1, Olennikov D.N.1, Pavlova S.I.3
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Affiliations:
- Institute of General and Experimental Biology SB RAS
- Medical institute, Buryat State University named after DorzhiBanzarov
- Chuvash State University named after I.N. Ulyanov of the Ministry of Science and Higher Education of the Russian Federation
- Issue: Vol 27, No 4 (2024)
- Pages: 63-69
- Section: Problems of experimental biology and medicine
- URL: https://journals.rcsi.science/1560-9596/article/view/315980
- DOI: https://doi.org/10.29296/25877313-2024-04-09
- ID: 315980
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Abstract
Introduction. The development and introduction of new drugs with immunomodulatory effects is one of the priorities of modern medicine. Numerous studies have proven the immunotropic activity of individual substances isolated from medicinal plants (flavonoids, phenylpropanoids, polysaccharides, ecdysteroids, terpenoids, etc.). In this study, it is of interest to determine the immunomodulatory effect of individual substances isolated from Rhaponticum uniflorum (L.) DC.
The purpose of the study. Determine the immunomodulatory properties of 20-hydroxyecdysone, 5-О-caffeoylquinic acid and arabino-3,6-galactan, isolated from the aerial part of Rhaponticum uniflorum (L.) DC, in relation to the cellular and humoral components of the immune response during experimental immunosuppression caused by cyclophosphamide.
Material and methods. The experiments were performed on 100 male mice of the F1 line (CBAxC57Bl/6) weighing 18-20 g. Immunodeficiency was simulated by in traperitoneal administration of cyclophosphamide at a dose of 250 mg/kg once. The experimental group of animals received individual substances intragastricallyin the following doses: 5-О-caffeoylquinic acid - 10 mg/kg, arabino-3,6-galactan - 10 mg/kg, 20-hydroxyecdysone - 3 mg/kg, once a day for 14 days. The intact group of mice received purified water according to the analogous scheme. The state of humoral chain of the immune response was estimated according to the quantity of antibody-forming cells (AFC) determined with the use of Cunningham's plaque assay (1965). The state of the cellular chain of the immune response was estimated according to the delayed hypersensitivity reaction.
Study results. It has been established that 20-hydroxyecdysone, 5-О-caffeoylquinic acid and arabino-3,6-galactan, isolated from the aerial part of Rhaponticum uniflorum (L.) DC, have immunomodulatory effect on the cellular and humoral components of the immune response in immunosuppression caused by cyclophosphamide.
Conclusions. The obtained results argues the feasibility of further research of individual substances, isolated from the aerial part of Rhaponticum uniflorum (L.) DC, with a view to creating new effective immunomodulators of plant origin.
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##article.viewOnOriginalSite##About the authors
V. B. Khobrakova
Institute of General and Experimental Biology SB RAS; Medical institute, Buryat State University named after DorzhiBanzarov
Author for correspondence.
Email: val0808@mail.ru
Dr.Sc. (Biol.), Associate Professor, Head of the Laboratory of Experimental Pharmacology; Professor of the Department of Human General Pathology
Russian Federation, Ulan-Ude; Ulan-UdeYu. A. Tugarina
Institute of General and Experimental Biology SB RAS
Email: ytugarina@mail.ru
Post-graduate Student, Laboratory of Experimental Pharmacology
Russian Federation, Ulan-UdeD. N. Olennikov
Institute of General and Experimental Biology SB RAS
Email: olennikovdn@mail.ru
D.Sc (Pharm.), Head of the Laboratory of Biomedical Research
Russian Federation, Ulan-UdeS. I. Pavlova
Chuvash State University named after I.N. Ulyanov of the Ministry of Science and Higher Education of the Russian Federation
Email: flavonoid@yandex.ru
Dr.Sc. (Med.), Professor, Head of the Department of Pharmacology, Clinical Pharmacology and Biochemistry, Faculty of Medicine
Russian Federation, CheboksaryReferences
- Dotsenko E.A., Rozhdestvensky D.A., Yupatov G.I. Immunodeficiency and some immunomodulatory agents. Bulletin of VSMU. 2014; 3: 103–120.
- Jantan I., Ahmad W., Bukhari S.N. Plant-derived immunomodulators: an insight on their preclinical evaluation and clinical trials. Frontiers in Plant Science. 2015; 6: 655.
- Nagoor Meeran M.F., Javed H., Sharma C., et al. Can Echinacea be a potential candidate to target immunity, inflammation, and infection – The trinity of coronavirus disease. Heliyon. 2021; 7(2): 05990.
- Shur Yu.V., Shur V.Yu., Samotrueva M.A. Some mechanisms of immunotropic and adaptogenic action of phytopreparations. Reviews on clinical pharmacology and drug therapy. 2019; 17(4): 19–29.
- Provenza F.D., Villalba J.J. The role of natural plant products in modulating the immune system: An adaptable approach for combating disease in grazing animals. Small Ruminant Research. 2010; 89(2–3): 131–139.
- Stanescu V. Immunotherapeutic properties of medical plants. Rev.Med. Chir.Med.Nat. 2016; 50: 586–621.
- Koledaeva E.V., Panfilov A.B., Chashnikov D.D. Indicators of the lymphoid tissue of the intestinal wall of white mongrel nonlinear mice after the action of phytoecdysteroids of Rhaponticum scariosum extract. Hippology and veterinary medicine. 2021; 4(42): 82–87.
- Kurkin V.A. Phenylpropanoids as the most important group of biologically active compounds of medicinal plants. International Journal of Applied and Fundamental Research. 2015; 12(7): 1338–1342.
- Chauhan P.S., Satti N.K., Sharma P., et al. Differential effects of chlorogenic acid on various immunological parameters relevant to rheumatoid arthritis. Phytother Res. 2012; 26(8): 1156–1165.
- Hosseinzade A., Sadeghi O., NaghdipourBiregani A., et al. Immunomodulatory effects of flavonoids: possible induction of t CD4+ regulatory cells through suppression of mTOR pathway signaling activity. Frontiers in Immunology. 2019. 10: 51.
- Kzhyshkowska J. Stabilizing the immune system by chlorogenic acid. Journal of Leukocyte Biolog. 2022; 112(1): 7–8.
- Yu J., Cong L., Wang C. et al. Immunomodulatory effect of Schisandra polysaccharides in cyclophosphamide-induced immunocompromised mice. Experimental and Therapeutic Medicine. 2018; 15(6): 4755–4762.
- Khobrakova V. B., Tugarina Yu. A., Toropova A. A. i dr. The effect of dried single-flowered leucea extract on the state of the immune and antioxidant systems of the body in experimental immunodeficiency. Questions of biological, medical and pharmaceutical chemistry. 2022; 25(1): 43–49.
- Masnaya N.V., Borsuk O.S., SherstoboevE.Yu. i dr. Correction of cytostatic immunodeficiency with plant polyphenolic compounds. Bulletin of the Ural Medical Academic Science. 2011; 2/2(35): 44–45.
- Olennikov D.N. Minor ecdysteroids from Rhaponticum uniflorum leaves from Eastern Siberia. Chem. Nat. Compd. 2018; 54 (4): 798–800.
- Olennikov D.N., Kashchenko N.I. New flavonoids and turkesterone-2-O-cinnamate from leaves of Rhaponticum uniflorum. Chem. Nat. Compd. 2019; 55: 256–264.
- Olennikov D.N. Free carbohydrates, glucofructans, and other polysaccharides from Rhaponticum uniflorum. Chem. Nat. Comp. 2018; 54: 751–754.
- Mironov A.N. Guidelines for conducting preclinical studies of medicines, part 1. Moscow, 2012.
- Cunningham A.A. Method of Increased Sensitivity for detecting Single Antibody-forming Cells. Nature. 1965; 207: 1106–1107.
- Sergienko V.I., Bondareva I.B. Mathematical statistics in clinical research. Practical guide M.: GOETAR-Media, 2006. 304.
- Ma L., Jiao K., Luo L., et al. Characterization and macrophage immunomodulatory activity of two polysaccharides from the flowers of Paeonia suffruticosa Andr. International Journal of Biological Macromolecules. 2019; 124: 955–962
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