Molecular mechanisms of antibacterial action of the isolate of essential oils Kist-Al-Hindi (Saussurea costus (Falc.) Lipsch.) on some opportunistic representatives of human microflora
- Authors: Kuzminov I.V.1, Boyarintsev D.I.1, Gadzhiev G.K.1, Gogokhiya G.R.1, Bryutova K.V.1, Rodina Y.S.1
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Affiliations:
- Tyumen State Medical University
- Issue: Vol 29, No 1 (2026)
- Pages: 25-33
- Section: Pharmaceutical chemistry
- URL: https://journals.rcsi.science/1560-9596/article/view/375516
- DOI: https://doi.org/10.29296/25877313-2026-01-04
- EDN: https://elibrary.ru/YAGTUS
- ID: 375516
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Abstract
Introduction. Saussurea costus (Falc.) Lipsch. is a plant widely used in traditional medicine in Arab countries. Its use as a medicinal raw material is mentioned in the sacred hadiths of Islam. Its use has been suggested as an antibacterial and antiseptic agent, but to date, there is no definitive answer to the mechanism of antibacterial action of the phytochemical components of this plant.
The aim of this study was to obtain an essential oil isolate of Saussurea costus (Falc.) Lipsch. This isolate's chemical composition and determination of its antibacterial activity were analyzed. The mechanism of antibacterial action of essential oil components from this plant was also explored.
Material and Methods. One possible molecular mechanism of action of lactones, components of essential oils, on HMG-CoA reductase, a regulatory enzyme of the MVA cascade, is discussed. An essential oil fraction from the bark of Kist-al-Hindi was obtained by hydrodistillation after 180 minutes of fractional distillation. The component composition of the fractions was analyzed by gas chromatography. The composition of the fractions was assessed using a mass-spectrometric detector. Antibacterial activity was tested based on changes in the optical density of microorganisms cultured with the addition of essential oils. The data obtained from the antimicrobial activity screening were statistically processed, with results considered significant at p < 0.05.
Results. A relationship was identified between the metabolic profiles of opportunistic microorganisms of normal human microflora and the mechanism of action of compounds contained in the essential oil fraction from this plant. A mechanism for the antibacterial action of essential oil components on the MVA cascade of terpenoid chain biosynthesis is proposed. The essential oil of the bark of Saussurea costus (Falc.) Lipsch. Sesquiterpene lactones were detected: dihydrococtus lactone and costus lactone. The obtained optical density results (p < 0.0001) indicate suppression of the growth of some opportunistic bacteria of the normal human microflora.
Conclusion. This plant material has the potential to act as an antiseptic against opportunistic pathogens such as Candida albicans, Saccharomyces cerevisiae, Streptococcus mutans, and Enterococcus faecalis.
About the authors
I. V. Kuzminov
Tyumen State Medical University
Author for correspondence.
Email: kowendi3@gmail.com
ORCID iD: 0000-0002-0097-2219
SPIN-code: 6181-4600
Laboratory assistant, Laboratory of Metabolic and Cellular Engineering
Russian Federation, 54 Odesskaya str., Tyumen, 625023D. I. Boyarintsev
Tyumen State Medical University
Email: bdy0710@yandex.ru
ORCID iD: 0000-0002-5605-1849
SPIN-code: 9454-5994
Ph.D. (Biol.), Head of the Laboratory of Metabolic and Cellular Engineering, Associate Professor of the Department of Pharmaceutical Sciences
Russian Federation, 54 Odesskaya str., Tyumen, 625023G. K. Gadzhiev
Tyumen State Medical University
Email: gghhshdhsd@gmail.com
ORCID iD: 0009-0000-7383-352X
Student of the Institute of Pharmacy
Russian Federation, 54 Odesskaya str., Tyumen, 625023G. R. Gogokhiya
Tyumen State Medical University
Email: gogoxiya47@mail.ru
ORCID iD: 0009-0009-3895-0662
SPIN-code: 9525-7899
Student of the Institute of Pharmacy
Russian Federation, 54 Odesskaya str., Tyumen, 625023K. V. Bryutova
Tyumen State Medical University
Email: barmoglot19@mail.ru
Lecturer of the Department of Biology
Russian Federation, 54 Odesskaya str., Tyumen, 625023Yu. S. Rodina
Tyumen State Medical University
Email: RodinaYS@tyumsmu.ru
ORCID iD: 0000-0002-0612-3998
SPIN-code: 5774-4913
Ph.D. (Pharm.), Director of the Institute of Pharmacy
Russian Federation, 54 Odesskaya str., Tyumen, 625023References
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